Pietrantonio F, Noble P B, Amsel R, Chan E C
Department of Oral Biology, McGill University, Montréal, Qué., Canada.
Can J Microbiol. 1988 Jun;34(6):748-52. doi: 10.1139/m88-127.
Locomotion of pathogenic spirochetes has been suggested as a virulence factor in their pathogenesis. Little is known of the locomotory characteristics of oral anaerobic spirochetes. We have determined the optimal conditions for motility of seven strains of Treponema denticola in menstrua of different viscosities. The viscosity for optimum motility for all strains was found to be 9.57 centipoises at 25 degrees C. Under these conditions the average speeds for each strain was computed from the motility tracks as recorded by timed exposures under dark-field microscopy. Differences in speeds were found between the various strains. In addition, we have determined the "persistence" (direct distance/actual pathlength travelled) of cell movement of each strain. Interstrain differences were also noted. These locomotory characteristics contribute to the locomotory phenotypes of the various strains and therefore may aid in their characterization and provide an insight into locomotion as a virulence factor in periodontitis.
致病性螺旋体的运动已被认为是其发病机制中的一种毒力因子。人们对口腔厌氧螺旋体的运动特征知之甚少。我们已经确定了七株齿垢密螺旋体在不同粘度培养液中的最佳运动条件。发现所有菌株在25℃时最佳运动的粘度为9.57厘泊。在这些条件下，通过暗视野显微镜定时曝光记录的运动轨迹计算出每个菌株的平均速度。发现不同菌株之间的速度存在差异。此外，我们还确定了每个菌株细胞运动的“持续性”（直接距离/实际行进路径长度）。也注意到了菌株间的差异。这些运动特征有助于不同菌株的运动表型，因此可能有助于对它们进行表征，并深入了解运动作为牙周炎毒力因子的作用。
