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增强鞘脂合成可提高酿酒酵母的渗透压耐受性。

Enhancement of Sphingolipid Synthesis Improves Osmotic Tolerance of Saccharomyces cerevisiae.

机构信息

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China.

Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, China.

出版信息

Appl Environ Microbiol. 2020 Apr 1;86(8). doi: 10.1128/AEM.02911-19.

DOI:10.1128/AEM.02911-19
PMID:32033944
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7117927/
Abstract

To enhance the growth performance of under osmotic stress, mutant XCG001, which tolerates up to 1.5 M NaCl, was isolated through adaptive laboratory evolution (ALE). Comparisons of the transcriptome data of mutant XCG001 and the wild-type strain identified as being associated with osmotic tolerance. In the overexpression strain (XCG010), the contents of inositol phosphorylceramide (IPC; t18:0/26:0), mannosylinositol phosphorylceramide [MIPC; t18:0/22:0(2OH)], MIPC (d18:0/22:0), MIPC (d20:0/24:0), mannosyldiinositol phosphorylceramide [M(IP)C; d20:0/26:0], M(IP)C [t18:0/26:0(2OH)], and M(IP)C [d20:0/26:0(2OH)] increased by 88.3 times, 167 times, 63.3 times, 23.9 times, 27.9 times, 114 times, and 208 times at 1.0 M NaCl, respectively, compared with the corresponding values of the control strain XCG002. As a result, the membrane integrity, cell growth, and cell survival rate of strain XCG010 increased by 24.4% ± 1.0%, 21.9% ± 1.5%, and 22.1% ± 1.1% at 1.0 M NaCl, respectively, compared with the corresponding values of the control strain XCG002 (wild-type strain with a control plasmid). These findings provided a novel strategy for engineering complex sphingolipids to enhance osmotic tolerance. This study demonstrated a novel strategy for the manipulation of membrane complex sphingolipids to enhance tolerance to osmotic stress. Elo2, a sphingolipid acyl chain elongase, was related to osmotic tolerance through transcriptome analysis of the wild-type strain and an osmosis-tolerant strain generated from ALE. Overexpression of increased the content of complex sphingolipid with longer acyl chain; thus, membrane integrity and osmotic tolerance improved.

摘要

为了提高 在渗透胁迫下的生长性能，通过适应性实验室进化（ALE）分离出耐受高达 1.5 M NaCl 的突变体 XCG001。比较突变体 XCG001 和野生型菌株的转录组数据，鉴定出 与渗透耐受相关。在 过表达菌株（XCG010）中，肌醇磷酸神经酰胺（IPC；t18:0/26:0）、甘露糖基肌醇磷酸神经酰胺[MIPC；t18:0/22:0(2OH)]、MIPC（d18:0/22:0）、MIPC（d20:0/24:0）、甘露糖二肌醇磷酸神经酰胺[M（IP）C；d20:0/26:0]、M（IP）C [t18:0/26:0(2OH)] 和 M（IP）C [d20:0/26:0(2OH)] 的含量分别比对照菌株 XCG002 增加了 88.3 倍、167 倍、63.3 倍、23.9 倍、27.9 倍、114 倍和 208 倍在 1.0 M NaCl 下，结果，菌株 XCG010 的膜完整性、细胞生长和细胞存活率分别比对照菌株 XCG002（带有对照质粒的野生型菌株）增加了 24.4%±1.0%、21.9%±1.5%和 22.1%±1.1%。这些发现为工程复杂神经酰胺以提高渗透耐受性提供了一种新策略。本研究通过对野生型菌株和 ALE 产生的耐渗菌株的转录组分析，证明了一种通过操纵膜复合神经酰胺来提高 对渗透压应激的耐受性的新策略。Elo2 是一种鞘脂酰基链延长酶，通过对野生型菌株和耐渗菌株的转录组分析，发现它与渗透压耐受有关。 的过表达增加了具有更长酰链的复合神经酰胺的含量；因此，膜完整性和渗透压耐受性得到改善。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7506/7117927/d294aedc97b8/AEM.02911-19-f0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7506/7117927/7e851a773fb4/AEM.02911-19-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7506/7117927/3345c450e788/AEM.02911-19-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7506/7117927/2260bc4949df/AEM.02911-19-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7506/7117927/75df36d17921/AEM.02911-19-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7506/7117927/9c874e9d63a4/AEM.02911-19-f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7506/7117927/d294aedc97b8/AEM.02911-19-f0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7506/7117927/7e851a773fb4/AEM.02911-19-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7506/7117927/3345c450e788/AEM.02911-19-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7506/7117927/2260bc4949df/AEM.02911-19-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7506/7117927/75df36d17921/AEM.02911-19-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7506/7117927/9c874e9d63a4/AEM.02911-19-f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7506/7117927/d294aedc97b8/AEM.02911-19-f0006.jpg

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