从红龙果（Hylocereus polyrhizus）中分离到的贝纳病毒属一新成员的全基因组序列。
Complete genome sequence of a new member of the genus Badnavirus from red pitaya (Hylocereus polyrhizus).
机构信息
Institute of Pomology Science, Guizhou Provincial Academy of Agricultural Sciences, Huaxi District, Jinzhu Town, Guiyang, 550006, Guizhou, China.
出版信息
Arch Virol. 2020 Mar;165(3):749-752. doi: 10.1007/s00705-019-04503-7. Epub 2020 Feb 7.
Here, we report a circular double-stranded DNA virus from red pitaya (Hylocereus polyrhizus). The complete genome sequence is 7,837 nt in length and shares 98.7% nucleotide sequence identity with epiphyllum mottle-associated virus (EpMoaV) and 40.4-54.6% with other members of the genus Badnavirus. It has four open reading frames (ORFs), encoding putative proteins of 19.9, 14.8, 225.7 and 14.2 kDa, respectively. The reverse transcriptase (RT)-ribonuclease H (RNase H) region exhibits less than 70.5% nucleotide sequence identity to RT-RNase H of other badnaviruses, and 99.7% to that of EpMoaV. Phylogenetic analysis revealed that the virus from this study and EpMoaV form a single group. Consequently, we propose this virus as a new member of the genus Badnavirus in the family Caulimoviridae and have named it "pitaya badnavirus 1" (PiBV1). PiBV1 and EpMoaV should be considered two isolates of a badnavirus that infects members of the family Cactaceae.
在这里，我们报道了一种来自火龙果（Hylocereus polyrhizus）的环状双链 DNA 病毒。该病毒的完整基因组序列长 7837 个核苷酸，与 Epiphyllum mottle-associated virus（EpMoaV）的核苷酸序列同源性为 98.7%，与 Badnavirus 属的其他成员的同源性为 40.4-54.6%。它有四个开放阅读框（ORFs），分别编码推定的 19.9、14.8、225.7 和 14.2 kDa 的蛋白。逆转录酶（RT）-核糖核酸酶 H（RNase H）区的核苷酸序列与其他 badnaviruses 的 RT-RNase H 的同一性小于 70.5%，与 EpMoaV 的同一性为 99.7%。系统进化分析表明，本研究中的病毒与 EpMoaV 形成一个单独的组。因此，我们建议将该病毒作为 Caulimoviridae 科 Badnavirus 属的一个新成员，并将其命名为“火龙果 badnavirus 1”（PiBV1）。PiBV1 和 EpMoaV 应被视为感染仙人掌科成员的 badnavirus 的两个分离株。
Zotero 插件