Qabaha Khaled, Abbadi Jehad, Al-Rimawi Fuad
Department of Medical Laboratory Sciences, Faculty of Allied Health Sciences, Arab American University in Palestine, Jenin, Palestine.
Biology Department, Faculty of Science and Technology, Al-Quds University, P.O. Box 20002, Jerusalem, Palestine.
Afr Health Sci. 2025 Jun;25(2):330-342. doi: 10.4314/ahs.v25i2.39.
The Araliaceae family member Hedera helix L is well-known in traditional medicine for its ability to effectively treat a wide range of illnesses. This study uses High-Pressure Liquid Chromatography (HPLC) with a Photodiode array detector (PDA) detector to examine the phytochemical composition of the ethanolic extract obtained from Hedera helix leaves and explores its potential as an anti-inflammatory agent. Specifically, flavonoids and polyphenolic compounds are the focus of this analysis.
Ethanol was used to extract compounds from ivy leaves. Real-time reverse-transcriptase polymerase chain reaction (RT-PCR) was used to investigate its anti-inflammatory properties using lipopolysaccharide-pretreated white blood cells. Phytochemical analysis of the ivy leaf extract was conducted using the reversed-phase HPLC with PDA.
High-Pressure Liquid Chromatography of the ethanolic extract revealed the presence of numerous polyphenolic compounds, three of which were successfully identified as 4-Hydroxyphenyl acetic acid, rutin, and hesperidin. Furthermore, the extract demonstrated significant anti-inflammatory properties, notably inhibiting the gene expression of Interleukin-6.
The results of this investigation underscore the anti-inflammatory potential of Hedera helix extract. Various polyphenolic compounds and flavonoids were detected in the ethanolic extract, three of which were successfully identified. These compounds are presumed to be responsible for the observed activities. Further studies are necessary to elucidate the specific mechanisms of action.
五加科植物常春藤在传统医学中因其有效治疗多种疾病的能力而闻名。本研究使用配备光电二极管阵列检测器（PDA）的高压液相色谱（HPLC）来检测从常春藤叶中获得的乙醇提取物的植物化学成分，并探索其作为抗炎剂的潜力。具体而言，黄酮类化合物和多酚类化合物是该分析的重点。
用乙醇从常春藤叶中提取化合物。使用实时逆转录聚合酶链反应（RT-PCR），利用经脂多糖预处理的白细胞来研究其抗炎特性。采用带PDA的反相HPLC对常春藤叶提取物进行植物化学分析。
乙醇提取物的高压液相色谱分析显示存在多种多酚类化合物，其中三种被成功鉴定为4-羟基苯乙酸、芦丁和橙皮苷。此外，该提取物表现出显著的抗炎特性，尤其能抑制白细胞介素-6的基因表达。
本研究结果强调了常春藤提取物的抗炎潜力。在乙醇提取物中检测到了多种多酚类化合物和黄酮类化合物，其中三种被成功鉴定。推测这些化合物是观察到的活性的原因。需要进一步研究以阐明具体的作用机制。
