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高通量测序与食品微生物学。

High-throughput sequencing and food microbiology.

作者信息

Quijada Narciso M, Hernández Marta, Rodríguez-Lázaro David

机构信息

Laboratorio de Biología Molecular y Microbiología, Ibstituto tecnológico Agrario de Castilla y León (ITACyL), Valladolid, Spain.

Laboratorio de Biología Molecular y Microbiología, Ibstituto tecnológico Agrario de Castilla y León (ITACyL), Valladolid, Spain; Microbiology Division, Department of Biotechnology and Food Science, Faculty of Sciences, University of Burgos, Burgos, Spain.

出版信息

Adv Food Nutr Res. 2020;91:275-300. doi: 10.1016/bs.afnr.2019.10.003. Epub 2019 Nov 15.

Abstract

Massive parallel sequencing (High-Throughput Sequencing, HTS) permits reading of sequenced millions to billions short DNAs in parallel (reads) and is revolutionizing microbiology and food safety research from the laboratory methods to computational analysis, with the inevitable use of Bioinformatics. The time and cost reduction of microbiota, microbiome and metagenome studies allows the rapid progress in diagnosis, taxonomy, epidemiology, comparative genomics, virulence, discovery of genes or variants of interest and the association of microorganisms with food spoilage and foodborne infections.

摘要

大规模平行测序(高通量测序,HTS)允许并行读取数百万到数十亿条短DNA序列(读段),正彻底改变从实验室方法到计算分析的微生物学和食品安全研究,不可避免地要用到生物信息学。微生物群、微生物组和宏基因组研究在时间和成本上的降低,使得在诊断、分类学、流行病学、比较基因组学、毒力、发现感兴趣的基因或变体以及微生物与食品腐败和食源性感染的关联等方面取得快速进展。

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