Department of Operative Dentistry, Faculty of Dentistry, Chulalongkorn University.
Department of Microbiology and Research Unit on Oral Microbiology and Immunology, Faculty of Dentistry, Chulalongkorn University.
Dent Mater J. 2020 Jun 5;39(3):456-463. doi: 10.4012/dmj.2018-343. Epub 2020 Feb 7.
To investigate applications of chitosan as antibacterial agent for endodontic treatments, we tested its activity against Enterococcus faecalis standard strain (ATCC29212) and clinical isolates. We determined the minimum bactericidal concentration (MBC) of 6 types of chitosan against ATCC29212; the most effective types were selected for further tests. Four clinical isolates were cultured from endodontically treated-teeth and identified by biochemical assays and polymerase chain reactions. Bacterial cultures were exposed to 1,700 and 2,100 kDa chitosan at MBC for 1, 3, 5, 10, and 60 min in time-kill assays and plated on brain-heart-infusion (BHI) agar for colony counts. Both types of chitosan showed significantly lower numbers of remaining bacteria (log colony forming units per millimeter, logCFUs/mL) than negative controls (0.1% acetic acid and BHI) at 10 min, and completely eliminated the bacteria at 60 min for all strains. Thus, chitosan could be developed as alternative biocompatible antimicrobial irrigant/medication for endodontic treatments.
为了研究壳聚糖作为根管治疗抗菌剂的应用，我们测试了其对粪肠球菌标准株（ATCC29212）和临床分离株的活性。我们测定了 6 种壳聚糖对 ATCC29212 的最低杀菌浓度（MBC）；选择最有效的类型进行进一步测试。从根管治疗的牙齿中培养了 4 株临床分离株，并通过生化试验和聚合酶链反应进行了鉴定。在杀菌试验中，将细菌培养物暴露于 MBC 的 1700 和 2100 kDa 壳聚糖中 1、3、5、10 和 60 分钟，然后将其接种于脑心浸出液（BHI）琼脂上进行菌落计数。在 10 分钟时，与阴性对照（0.1%乙酸和 BHI）相比，这两种类型的壳聚糖都显著减少了剩余细菌的数量（每毫米对数形成单位，logCFUs/mL），并且所有菌株在 60 分钟时都完全消除了细菌。因此，壳聚糖可以开发为替代的生物相容性抗菌冲洗液/药物，用于根管治疗。
