antibacterial effect of a nano-zinc oxide eugenol sealer alone and in combination with chitosan, propolis, and nanosilver on .

BACKGROUND

This study aimed to assess the antibacterial effect of a nano-zinc oxide eugenol (nZOE) sealer alone and in combination with chitosan, propolis, and nanosilver on .

MATERIALS AND METHODS

In this , experimental study, nanosilver, chitosan, and propolis with 10wt%, 20wt%, and 60wt% concentrations, respectively, were added to nZOE sealer, and their antibacterial activity against was evaluated by agar diffusion and broth microdilution tests. The diameter of the growth inhibition zones was measured, and the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values were calculated for all materials. Data were analyzed by -test (alpha = 0.05).

RESULTS

The addition of nanosilver, chitosan, and propolis to nZOE did not change the diameter of growth inhibition zone in agar diffusion test. Propolis and eugenol alone showed the lowest MIC and MBC. Chitosan alone showed the highest MIC and MBC. Furthermore, nZOE showed lower MBC than micro-ZOE ( = 0.000). All groups containing nZOE showed the lowest MIC and MBC values.

CONCLUSION

The addition of propolis to nZOE can enhance its antibacterial activity against .