Ugalmugale Snehal Ramnath, Bohora Aarti Ashok, Patel Pawan Ashok, Sharma Vinay, Sengupta Shubhra, Sharma Sneha Monesh
Department of Conservative Dentistry and Endodontics, SMBT Institute of Dental Sciences and Research, Nashik, Maharashtra, India.
Department of Microbiology, SMBT Institute of Medical Sciences and Research Centre, Nashik, Maharashtra, India.
J Conserv Dent Endod. 2024 Aug;27(8):801-806. doi: 10.4103/JCDE.JCDE_172_24. Epub 2024 Aug 7.
The objective is to evaluate and compare the antibacterial efficacy of nitrofurantoin, chitosan, and calcium hydroxide (Ca(OH)2) in combination with propylene glycol (PG) as an intracanal medicament against endodontic pathogens.
Fifty-two extracted single-rooted maxillary and mandibular anterior teeth and premolars were selected. The root canals were enlarged using Protaper universal rotary files. Clinical isolates of microorganisms collected from retreatment cases were used. Bacterial isolates obtained from infected root canals were introduced into brain-heart infusion (BHI) broth. Incubation of samples for 14 days was carried out to facilitate the development of mature biofilms. Intracanal medicaments were divided into four groups: Group 1 - Nirofurantoin+20% PG, Group 2 - Chitosan+20% PG, Group 3 - Ca(OH)2 + 20% PG, and Group 4 - 20% PG. The prepared root samples were incubated for 7 days. After collecting dentin samples, they were placed in a phosphate-buffered saline solution. Serial dilutions were then performed, and each dilution was plated on BHI agar. The plates were incubated for 24 h at 37°C. The antibacterial efficacy was assessed by calculating the percentage of remaining colony-forming units.
Antibacterial efficacy of chitosan paste was significantly higher followed by nitrofurantoin as compared to other groups when used as an intracanal medicament.
In the root-canal biofilm model, the combination of chitosan and PG demonstrated a significant reduction in the viability of endodontic pathogens when employed as intracanal medication for 7 days. This suggests its potential as an effective intracanal medicament for endodontic retreatment.
评估并比较呋喃妥因、壳聚糖以及氢氧化钙（Ca(OH)₂）与丙二醇（PG）联合作为根管内药物对牙髓病原菌的抗菌效果。
选取52颗拔除的单根上颌和下颌前牙及前磨牙。使用Protaper通用旋转锉扩大根管。采用从再治疗病例中收集的微生物临床分离株。将从感染根管中获得的细菌分离株接种于脑心浸液（BHI）肉汤中。对样本进行14天的培养以促进成熟生物膜的形成。根管内药物分为四组：第1组 - 呋喃妥因 + 20% PG，第2组 - 壳聚糖 + 20% PG，第3组 - Ca(OH)₂ + 20% PG，第4组 - 20% PG。将制备好的牙根样本培养7天。收集牙本质样本后，将其置于磷酸盐缓冲盐溶液中。然后进行系列稀释，并将每个稀释液接种于BHI琼脂平板上。平板在37°C下培养24小时。通过计算剩余菌落形成单位的百分比来评估抗菌效果。
壳聚糖糊剂作为根管内药物使用时，其抗菌效果显著高于其他组，其次是呋喃妥因。
在根管生物膜模型中，壳聚糖与PG联合作为根管内药物使用7天时，牙髓病原菌的活力显著降低。这表明其作为牙髓再治疗有效根管内药物的潜力。
