文献检索文档翻译深度研究
Suppr Zotero 插件Zotero 插件
邀请有礼套餐&价格历史记录

新学期，新优惠

限时优惠:9月1日-9月22日

30天高级会员仅需29元

1天体验卡首发特惠仅需5.99元

了解详情
不再提醒
插件&应用
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
高级版
套餐订阅购买积分包
AI 工具
文献检索文档翻译深度研究
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2025

人参皂苷 Rg1 通过激活沉默调节蛋白 1（SIRT1）/结节性硬化复合物 2（TSC2）信号通路抑制来源于 KG1α 急性髓系白血病细胞的 CD34+CD38- 白血病干细胞的增殖并诱导其衰老标志物的表达。

Ginsenoside Rg1 Inhibits Cell Proliferation and Induces Markers of Cell Senescence in CD34+CD38- Leukemia Stem Cells Derived from KG1α Acute Myeloid Leukemia Cells by Activating the Sirtuin 1 (SIRT1)/Tuberous Sclerosis Complex 2 (TSC2) Signaling Pathway.

机构信息

Department of Radiology, The First Affiliated Hospital of Dali University, Dali, Yunnan, China (mainland).

Key Laboratory of Insect Biomedicine Research and Development in Yunnan Province, Dali University, Dali, Yunnan, China (mainland).

出版信息

Med Sci Monit. 2020 Feb 10;26:e918207. doi: 10.12659/MSM.918207.

DOI:10.12659/MSM.918207
PMID:32037392
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7032532/
Abstract

BACKGROUND Clinical relapse in acute myeloid leukemia (AML) is associated with the reduced treatment response of leukemia stem cells (LSCs). This study aimed to investigate the effects of the ginseng derivative, ginsenoside Rg1 (Rg1), on CD34+CD38- LSCs derived from KG1a human acute myeloid leukemia cells. MATERIAL AND METHODS CD34+CD38- LSCs were isolated from KG1a human acute myeloid leukemia cells by cell sorting. CD34+CD38- KG1alpha LSCs were divided into the control group and the Rg1 group (treated with Rg1). The cell counting kit-8 (CCK-8) assay evaluated the proliferation of CD34+CD38- KG1alpha LSCs and flow cytometry studied the cell cycle. The mixed colony-forming unit (CFU-Mix) assay and staining for senescence-associated beta-galactosidase (SA-ß-Gal) evaluated cell senescence. Expression of sirtuin 1 (SIRT1) and tuberous sclerosis complex 2 (TSC2) were evaluated using Western blot and quantitative reverse transcription-polymerase chain reaction (qRT-PCR). RESULTS CD34+CD38- KG1alpha LSCs were isolated at 98.72%. Rg1 significantly reduced the proliferation of CD34+CD38- KG1alpha LSCs compared with the control group (p<0.05). Cells in the G0/G1 phase were significantly increased, and cells in the G2/M and S phase were significantly reduced compared with the control group (p<0.05). Rg1 significantly increased SA-ß-Gal and reduced CFU-Mix formation compared with the control group (p<0.05), significantly down-regulated SIRT1 expression in CD34+CD38- KG1alpha LSCs compared with the control group (p<0.05), and significantly reduced TSC2 expression in CD34+CD38- KG1alpha LSCs compared with the control group (p<0.05). CONCLUSIONS Rg1 inhibited cell proliferation and induced cell senescence markers in CD34+CD38- KG1alpha LSCs by activating the SIRT1/TSC2 signaling pathway.

摘要

背景：急性髓系白血病（AML）的临床复发与白血病干细胞（LSCs）的治疗反应降低有关。本研究旨在探讨人参衍生物人参皂苷 Rg1（Rg1）对来源于 KG1a 人急性髓系白血病细胞的 CD34+CD38-LSCs 的影响。

材料与方法：通过细胞分选从 KG1a 人急性髓系白血病细胞中分离 CD34+CD38-LSCs。将 CD34+CD38-KG1alpha LSCs 分为对照组和 Rg1 组（用 Rg1 处理）。细胞计数试剂盒-8（CCK-8）检测 CD34+CD38-KG1alpha LSCs 的增殖，流式细胞术研究细胞周期。混合集落形成单位（CFU-Mix）检测和衰老相关β-半乳糖苷酶（SA-ß-Gal）染色评估细胞衰老。采用 Western blot 和实时定量聚合酶链反应（qRT-PCR）检测沉默信息调节因子 1（SIRT1）和结节性硬化复合物 2（TSC2）的表达。

结果：CD34+CD38-KG1alpha LSCs 的分离率为 98.72%。与对照组相比，Rg1 显著降低 CD34+CD38-KG1alpha LSCs 的增殖（p<0.05）。与对照组相比，G0/G1 期细胞明显增加，G2/M 和 S 期细胞明显减少（p<0.05）。与对照组相比，Rg1 显著增加 SA-ß-Gal 并减少 CFU-Mix 形成（p<0.05），显著下调 CD34+CD38-KG1alpha LSCs 中的 SIRT1 表达（p<0.05），并显著降低 CD34+CD38-KG1alpha LSCs 中的 TSC2 表达（p<0.05）。

结论：Rg1 通过激活 SIRT1/TSC2 信号通路抑制 CD34+CD38-KG1alpha LSCs 的细胞增殖并诱导细胞衰老标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d912/7032532/11d601560ff6/medscimonit-26-e918207-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d912/7032532/f713feaf9a44/medscimonit-26-e918207-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d912/7032532/361e40042303/medscimonit-26-e918207-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d912/7032532/c54c2f357ac4/medscimonit-26-e918207-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d912/7032532/810815f9b46d/medscimonit-26-e918207-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d912/7032532/11d601560ff6/medscimonit-26-e918207-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d912/7032532/f713feaf9a44/medscimonit-26-e918207-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d912/7032532/361e40042303/medscimonit-26-e918207-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d912/7032532/c54c2f357ac4/medscimonit-26-e918207-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d912/7032532/810815f9b46d/medscimonit-26-e918207-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d912/7032532/11d601560ff6/medscimonit-26-e918207-g005.jpg

相似文献

[1]
Ginsenoside Rg1 Inhibits Cell Proliferation and Induces Markers of Cell Senescence in CD34+CD38- Leukemia Stem Cells Derived from KG1α Acute Myeloid Leukemia Cells by Activating the Sirtuin 1 (SIRT1)/Tuberous Sclerosis Complex 2 (TSC2) Signaling Pathway.

Med Sci Monit. 2020-2-10

[2]
[Ginsenoside Rg_1 induces leukemia stem cell senescence via SIRT1/TSC_2 signal axis].

Zhongguo Zhong Yao Za Zhi. 2019-6

[3]
Ginsenoside Rg1 induces senescence of leukemic stem cells by upregulating p16INK4a and downregulating hTERT expression.

Adv Clin Exp Med. 2021-6

[4]
Senescence effects of Angelica sinensis polysaccharides on human acute myelogenous leukemia stem and progenitor cells.

Asian Pac J Cancer Prev. 2014-1

[5]
Lower phosphorylation of p38 MAPK blocks the oxidative stress-induced senescence in myeloid leukemic CD34(+)CD38 (-) cells.

J Huazhong Univ Sci Technolog Med Sci. 2012-6

[6]
Phenotyping and Target Expression Profiling of CD34/CD38 and CD34/CD38 Stem- and Progenitor cells in Acute Lymphoblastic Leukemia.

Neoplasia. 2018-5-15

[7]
SIRT6/NF-κB signaling axis in ginsenoside Rg1-delayed hematopoietic stem/progenitor cell senescence.

Int J Clin Exp Pathol. 2015-5-1

[8]
Live kinase B1 maintains CD34CD38 AML cell proliferation and self-renewal.

Mol Cell Biochem. 2017-4-10

[9]
CD45CD34CD38CD133 cells have the potential as leukemic stem cells in acute myeloid leukemia.

BMC Cancer. 2020-4-6

[10]
Resveratrol-downregulated phosphorylated liver kinase B1 is involved in senescence of acute myeloid leukemia stem cells.

J Huazhong Univ Sci Technolog Med Sci. 2015-8

引用本文的文献

[1]
Anti-leukemia effects of ginsenoside monomer: A narrative review of pharmacodynamics study.

Curr Ther Res Clin Exp. 2024-2-25

[2]
Using CADD tools to inhibit the overexpressed genes FAP, FN1, and MMP1 by repurposing ginsenoside C and Rg1 as a treatment for oral cancer.

Front Mol Biosci. 2023-10-23

[3]
Role of SIRT1 in Chemoresistant Leukemia.

Int J Mol Sci. 2023-9-23

[4]
Signaling pathways governing the behaviors of leukemia stem cells.

Genes Dis. 2023-3-23

[5]
Ginsenosides on stem cells fate specification-a novel perspective.

Front Cell Dev Biol. 2023-7-5

[6]
RUNX3 mediates keloid fibroblast proliferation through deacetylation of EZH2 by SIRT1.

BMC Mol Cell Biol. 2022-12-7

[7]
The role of protein acetylation in carcinogenesis and targeted drug discovery.

Front Endocrinol (Lausanne). 2022

[8]
A statistical network pre-processing method to improve relevance and significance of gene lists in microarray gene expression studies.

BMC Bioinformatics. 2022-9-27

[9]
Ginsenoside Rg1 suppresses cancer cell proliferation through perturbing mitotic progression.

J Ginseng Res. 2022-5

[10]
Nicotinamide as a Foundation for Treating Neurodegenerative Disease and Metabolic Disorders.

Curr Neurovasc Res. 2021

本文引用的文献

[1]
Transient receptor potential ankyrin 1 protects against sepsis-induced kidney injury by modulating mitochondrial biogenesis and mitophagy.

Am J Transl Res. 2018-12-15

[2]
DUSP facilitates RPMI8226 myeloma cell aging and inhibited TLR4 expression.

Eur Rev Med Pharmacol Sci. 2018-9

[3]
The regulation of DLTA gene in bacterial growth and biofilm formation by Parvimonas micra.

Eur Rev Med Pharmacol Sci. 2018-7

[4]
Autophagy mediators (FOXO1, SESN3 and TSC2) in Lewy body disease and aging.

Neurosci Lett. 2018-9-25

[5]
Ginsenoside Rg1 ameliorates testicular senescence changes in D‑gal‑induced aging mice via anti‑inflammatory and antioxidative mechanisms.

Mol Med Rep. 2018-3-1

[6]
High EVI1 Expression Predicts Poor Outcomes in Adult Acute Myeloid Leukemia Patients with Intermediate Cytogenetic Risk Receiving Chemotherapy.

Med Sci Monit. 2018-2-6

[7]
Alleviation of ginsenoside Rg1 on hematopoietic homeostasis defects caused by lead-acetate.

Biomed Pharmacother. 2017-11-11

[8]
Cooperative effect of chidamide and chemotherapeutic drugs induce apoptosis by DNA damage accumulation and repair defects in acute myeloid leukemia stem and progenitor cells.

Clin Epigenetics. 2017-8-14

[9]
Moving to the Rhythm with Clock (Circadian) Genes, Autophagy, mTOR, and SIRT1 in Degenerative Disease and Cancer.

Curr Neurovasc Res. 2017

[10]
Sirtuin 1 attenuates inflammation and hepatocellular damage in liver transplant ischemia/Reperfusion: From mouse to human.

Liver Transpl. 2017-10

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

推荐工具

医学文档翻译智能文献检索