Revie D, Tseng B Y, Grafstrom R H, Goulian M
Proc Natl Acad Sci U S A. 1979 Nov;76(11):5539-43. doi: 10.1073/pnas.76.11.5539.
The double-stranded replicative form (RF) DNA of the autonomous parvovirus H-1 can be isolated from infected cells in a covalent complex with protein. The protein is present on most or all of the RF DNA, including actively replicating molecules, and is associated with the 5'-terminal endonuclease Hae III fragments of both the viral and complementary strands of RF. The size of the protein is estimated to be 60,000-70,000 daltons from its effect on buoyant density of DNA. DNA with covalently bound protein has not been found in H-1 virions.
自主性细小病毒H-1的双链复制型（RF）DNA可从感染细胞中分离出来，呈与蛋白质的共价复合物形式。该蛋白质存在于大多数或所有的RF DNA上，包括活跃复制的分子，并与RF病毒链和互补链的5'-末端核酸酶Hae III片段相关联。根据其对DNA浮力密度的影响，估计该蛋白质的大小为60,000-70,000道尔顿。在H-1病毒粒子中未发现有共价结合蛋白质的DNA。
