Shaikh Abusufyan, Ibrahim Mohammed, Khan Mohib
School of Pharmacy, Anjuman-I-Islam's Kalsekar Technical Campus, New Panvel, Maharashtra, affiliated to Mumbai University, Mumbai, India.
Research Scholar, JNTUH, Kukatpally, Telangana, India.
EXCLI J. 2020 Jan 3;19:33-47. doi: 10.17179/excli2019-1441. eCollection 2020.
Diabetes mellitus is a complex metabolic disorder that leads to various micro-vascular complications. The present study elucidated the effect of chloroform extract of leaves of (CHFG) in streptozotocin-induced early diabetic renal and neural complications. Wistar rats were injected with STZ (55 mg/kg, i.p.) to produce experimental diabetes. Two weeks after the stabilization of diabetes, CHFG extract at the dose of 200 and 400 mg/kg (CHFG 200 and CHFG 400) and metformin at the dose of 250 mg/kg (Met 250) was administered to the diabetic rats for further two weeks. Diabetic rats showed an increase in blood glucose, plasma urea, uric acid, creatinine, triglyceride, and total cholesterol level. The change in behavioral parameters such as thermal hyperalgesia and cold allodynia with compromised sciatic nerve and kidney antioxidant status were seen in diabetic rats. Diabetic rats treated with CHFG 200, CHFG 400, and Met 250 showed a decrease in blood glucose, plasma urea, uric acid, creatinine, triglyceride, and total cholesterol level. Also, it improved altered behavioral parameters such as thermal hyperalgesia and cold allodynia. It also restored the sciatic nerve and kidney antioxidant status. The results of kidney and sciatic nerves histopathological study were in line with the results of biochemical parameters that confirmed the favorable role of CHFG. Characterization of CHFG by LC-MS revealed the presence of diverse phytoconstituents, which might be responsible for its protective effect.
糖尿病是一种复杂的代谢紊乱疾病，可导致各种微血管并发症。本研究阐明了[植物名称]叶氯仿提取物（CHFG）对链脲佐菌素诱导的早期糖尿病肾和神经并发症的影响。给Wistar大鼠腹腔注射链脲佐菌素（55mg/kg）以诱导实验性糖尿病。糖尿病病情稳定两周后，给糖尿病大鼠分别灌胃200mg/kg和400mg/kg剂量的CHFG提取物（CHFG 200和CHFG 400）以及250mg/kg剂量的二甲双胍（Met 250），持续两周。糖尿病大鼠的血糖、血浆尿素、尿酸、肌酐、甘油三酯和总胆固醇水平升高。糖尿病大鼠出现行为参数改变，如热痛觉过敏和冷觉异常，同时坐骨神经和肾脏抗氧化状态受损。用CHFG 200、CHFG 400和Met 250治疗的糖尿病大鼠血糖、血浆尿素、尿酸、肌酐、甘油三酯和总胆固醇水平降低。此外，它改善了热痛觉过敏和冷觉异常等行为参数改变。它还恢复了坐骨神经和肾脏的抗氧化状态。肾脏和坐骨神经组织病理学研究结果与生化参数结果一致，证实了CHFG的有益作用。通过液相色谱 - 质谱联用（LC-MS）对CHFG进行表征，发现存在多种植物成分，这可能是其具有保护作用的原因。
