Li Jie, Liu Qin, Huang Xuan, Cai Yurui, Song Li, Xie Qianrong, Liu Fuchuan, Chen Xiaochun, Xu Peng, Zeng Fanwei, Chu Yanpeng, Zeng Fanxin
Department of Clinical Research Center, Dazhou Central Hospital, Dazhou, China.
Medical Research Center, Beijing Chaoyang Hospital, Capital Medical University, Beijing, China.
Front Genet. 2020 Jan 21;10:1360. doi: 10.3389/fgene.2019.01360. eCollection 2019.
C-X-C motif chemokine ligand 8 () is involved in tumor proliferation, migration, and invasion. However, the function of in colorectal cancer (CRC) is controversial. Here, we analyzed RNA-sequencing (RNA-seq) data to identify differentially expressed genes and pathways according to gene ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways associated with CRC. The levels of the mRNA encoding were significantly increased in early and advanced stages of CRC, as well as in metastases and nonmetastasis cases using RNA-seq analysis (n = 91). These findings were consistent with immunohistochemical analysis of expression (n = 87). Protein-protein interaction (PPI) prediction combined with transcriptional profiling data revealed that levels positively correlated with cAMP responsive element binding protein 1 ()/ribosomal protein S6 kinase B1 () expression, which promotes cell proliferation and differentiation in high expression, while inversely correlated with the expression of Bcl2 associated agonist of cell death () protein to inhibit apoptosis during the progression of CRC. These findings provide compelling clinical and molecular evidence to support the conclusion that contributes to the genesis and progression of CRC.
C-X-C基序趋化因子配体8（）参与肿瘤增殖、迁移和侵袭。然而，其在结直肠癌（CRC）中的功能存在争议。在此，我们分析了RNA测序（RNA-seq）数据，以根据基因本体论（GO）富集以及与CRC相关的京都基因与基因组百科全书（KEGG）通路来鉴定差异表达基因和通路。使用RNA-seq分析（n = 91）发现，在CRC的早期和晚期阶段以及转移和非转移病例中，编码的mRNA水平均显著升高。这些发现与对表达的免疫组织化学分析结果一致（n = 87）。蛋白质-蛋白质相互作用（PPI）预测结合转录谱数据显示，水平与cAMP反应元件结合蛋白1（）/核糖体蛋白S6激酶B1（）的表达呈正相关，后者在高表达时促进细胞增殖和分化，而在CRC进展过程中与细胞死亡相关的Bcl2激动剂（）蛋白的表达呈负相关，以抑制细胞凋亡。这些发现提供了令人信服的临床和分子证据，支持参与CRC发生和进展的结论。
