• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

FOXS1通过上调结直肠癌中的CXCL8促进肿瘤进展。

FOXS1 Promotes Tumor Progression by Upregulating CXCL8 in Colorectal Cancer.

作者信息

Qiu Junfeng, Li Mingzhou, Su Cailin, Liang Yihao, Ou Ruizhang, Chen Xiaoning, Huang Chengmei, Zhang Yaxin, Ye Yaping, Liao Wenting, Zhang Chao

机构信息

Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China.

出版信息

Front Oncol. 2022 Jul 8;12:894043. doi: 10.3389/fonc.2022.894043. eCollection 2022.

DOI:10.3389/fonc.2022.894043
PMID:35898871
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9309265/
Abstract

BACKGROUND

Forkhead box S1 (FOXS1) is a member of the forkhead box (FOX) transcriptional factor superfamily. The biological roles and underlying regulatory mechanism of FOXS1 in CRC remain unclear.

METHODS

Bioinformatics analysis, Western blotting, real-time PCR, and immunohistochemistry (IHC) were used to detect the expression FOXS1 in CRC. MTT assay, transwell assay, human umbilical vein endothelial cell tube formation assay, and chicken chorioallantoic membrane assay were performed to investigate the effects of FOXS1 on proliferation, invasion, and angiogenesis. Additionally, tumor formation assay and orthotopic implantation assay were used to investigate the effects of FOXS1 on tumor growth and metastasis . Furthermore, gene set enrichment analysis (GSEA) was used to analyze the correlation between FOXS1 and EMT or angiogenesis. The correlation between FOXS1 and CXCL8 expression was analyzed in clinical CRC samples using IHC.

RESULTS

The results showed that FOXS1 expression was upregulated in CRC tissues compared with adjacent normal intestine tissues. A high FOXS1 expression is positively correlated with poor survival. FOXS1 promoted the malignant behavior of CRC cancer cells , including proliferation, invasion, and angiogenesis. In addition, FOXS1 promoted tumor growth and metastasis in nude mice. Mechanistically, FOXS1 upregulated the expression of C-X-C motif chemokine ligand 8 (CXCL8) at the transcriptional level. Knockdown of CXCL8 blocked FOXS1 induced the enhancement of the EMT and angiogenesis. GSEAs in public CRC datasets revealed strong correlations between FOXS1 expression and EMT marker and angiogenesis markers. IHC showed that FOXS1 expression was positively correlated with CXCL8 expression and CD31 expression in clinical CRC samples.

CONCLUSION

The results suggest that FOXS1 promotes angiogenesis and metastasis by upregulating CXCL8 in CRC. Interference with the FOXS1/CXCL8 axis may serve as a potential therapeutic target for the treatment of metastatic CRC.

摘要

背景

叉头框S1（FOXS1）是叉头框（FOX）转录因子超家族的成员。FOXS1在结直肠癌（CRC）中的生物学作用及潜在调控机制仍不清楚。

方法

采用生物信息学分析、蛋白质免疫印迹法、实时定量聚合酶链反应及免疫组织化学（IHC）检测CRC中FOXS1的表达。进行MTT法、Transwell法、人脐静脉内皮细胞管腔形成实验及鸡胚绒毛尿囊膜实验，以研究FOXS1对增殖、侵袭及血管生成的影响。此外，采用肿瘤形成实验及原位植入实验研究FOXS1对肿瘤生长和转移的影响。进一步地，运用基因集富集分析（GSEA）分析FOXS1与上皮-间质转化（EMT）或血管生成之间的相关性。使用IHC分析临床CRC样本中FOXS1与CXC趋化因子配体8（CXCL8）表达之间的相关性。

结果

结果显示，与相邻正常肠组织相比，CRC组织中FOXS1表达上调。FOXS1高表达与较差的生存率呈正相关。FOXS1促进CRC癌细胞的恶性行为，包括增殖、侵袭及血管生成。此外，FOXS1促进裸鼠肿瘤生长和转移。机制上，FOXS1在转录水平上调CXC基序趋化因子配体8（CXCL8）的表达。敲低CXCL8可阻断FOXS1诱导的EMT及血管生成增强。公共CRC数据集中的GSEA显示FOXS1表达与EMT标志物及血管生成标志物之间存在强相关性。IHC显示，临床CRC样本中FOXS1表达与CXCL8表达及CD31表达呈正相关。

结论

结果表明，FOXS1通过上调CRC中的CXCL8促进血管生成和转移。干扰FOXS1/CXCL8轴可能成为转移性CRC治疗的潜在靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b6a/9309265/9f103f5b70ac/fonc-12-894043-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b6a/9309265/dcff933a9260/fonc-12-894043-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b6a/9309265/5ac0efc2e884/fonc-12-894043-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b6a/9309265/20993c90f06a/fonc-12-894043-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b6a/9309265/a2b6f6e50956/fonc-12-894043-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b6a/9309265/a83d5164f25a/fonc-12-894043-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b6a/9309265/9f103f5b70ac/fonc-12-894043-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b6a/9309265/dcff933a9260/fonc-12-894043-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b6a/9309265/5ac0efc2e884/fonc-12-894043-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b6a/9309265/20993c90f06a/fonc-12-894043-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b6a/9309265/a2b6f6e50956/fonc-12-894043-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b6a/9309265/a83d5164f25a/fonc-12-894043-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b6a/9309265/9f103f5b70ac/fonc-12-894043-g006.jpg

相似文献

1
FOXS1 Promotes Tumor Progression by Upregulating CXCL8 in Colorectal Cancer.FOXS1通过上调结直肠癌中的CXCL8促进肿瘤进展。
Front Oncol. 2022 Jul 8;12:894043. doi: 10.3389/fonc.2022.894043. eCollection 2022.
2
Forkhead Box S1 mediates epithelial-mesenchymal transition through the Wnt/β-catenin signaling pathway to regulate colorectal cancer progression.叉头框蛋白 S1 通过 Wnt/β-连环蛋白信号通路介导上皮-间充质转化，调节结直肠癌细胞的进展。
J Transl Med. 2022 Jul 21;20(1):327. doi: 10.1186/s12967-022-03525-1.
3
CREB5 promotes invasiveness and metastasis in colorectal cancer by directly activating MET.CREB5 通过直接激活 MET 促进结直肠癌的侵袭和转移。
J Exp Clin Cancer Res. 2020 Aug 25;39(1):168. doi: 10.1186/s13046-020-01673-0.
4
Forkhead Box S1 Inhibits the Progression of Hepatocellular Carcinoma.叉头框蛋白S1抑制肝细胞癌的进展。
Onco Targets Ther. 2020 Nov 17;13:11839-11848. doi: 10.2147/OTT.S272596. eCollection 2020.
5
FOXS1 is regulated by GLI1 and miR-125a-5p and promotes cell proliferation and EMT in gastric cancer.FOXS1 受 GLI1 和 miR-125a-5p 的调控，促进胃癌细胞的增殖和 EMT。
Sci Rep. 2019 Mar 27;9(1):5281. doi: 10.1038/s41598-019-41717-w.
6
FOXS1 promotes prostate cancer progression through the Hedgehog/Gli1 pathway.FOXS1 通过 Hedgehog/Gli1 通路促进前列腺癌进展。
Biochem Pharmacol. 2023 Dec;218:115893. doi: 10.1016/j.bcp.2023.115893. Epub 2023 Oct 27.
7
Overexpression of FOXS1 in gastric cancer cell lines inhibits proliferation, metastasis, and epithelial-mesenchymal transition of tumor through downregulating wnt/β-catenin pathway.FOXS1 在胃癌细胞系中的过表达通过下调 wnt/β-catenin 通路抑制肿瘤的增殖、转移和上皮-间充质转化。
J Cell Biochem. 2019 Mar;120(3):2897-2907. doi: 10.1002/jcb.26821. Epub 2018 Nov 30.
8
Forkhead box K2 promotes human colorectal cancer metastasis by upregulating ZEB1 and EGFR.叉头框蛋白K2通过上调锌指E盒结合蛋白1（ZEB1）和表皮生长因子受体（EGFR）促进人类结直肠癌转移。
Theranostics. 2019 May 31;9(13):3879-3902. doi: 10.7150/thno.31716. eCollection 2019.
9
Circ_0026344 restrains metastasis of human colorectal cancer cells via miR-183.环状 RNA 0026344 通过 miR-183 抑制人结直肠癌细胞的转移。
Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):4038-4045. doi: 10.1080/21691401.2019.1669620.
10
CCL20 and CXCL8 synergize to promote progression and poor survival outcome in patients with colorectal cancer by collaborative induction of the epithelial-mesenchymal transition.CCL20 和 CXCL8 通过协同诱导上皮-间充质转化促进结直肠癌患者的进展和不良生存结局。
Cancer Lett. 2014 Jun 28;348(1-2):77-87. doi: 10.1016/j.canlet.2014.03.008. Epub 2014 Mar 18.

引用本文的文献

1
Three-Dimensional Culture of Orbital Fibroblasts From Thyroid Eye Disease Induce In Vivo-Like Tissue Remodeling and Fibrosis.来自甲状腺眼病的眼眶成纤维细胞的三维培养诱导体内样组织重塑和纤维化。
Invest Ophthalmol Vis Sci. 2025 Jun 2;66(6):67. doi: 10.1167/iovs.66.6.67.
2
Uncovering the Effects and Molecular Mechanisms of Shaoyao Decoction Against Colorectal Cancer Using Network Pharmacology Analysis Coupled With Experimental Validation and Gut Microbiota Analysis.基于网络药理学分析结合实验验证及肠道微生物群分析揭示芍药汤抗结直肠癌的作用及分子机制
Cancer Med. 2025 Mar;14(6):e70813. doi: 10.1002/cam4.70813.
3
New Perspectives in Colorectal Cancers Treatment, the Role of MicroRNAs.

本文引用的文献

1
TGFβ-induced FOXS1 controls epithelial-mesenchymal transition and predicts a poor prognosis in liver cancer.TGFβ 诱导的 FOXS1 控制上皮-间充质转化，并预测肝癌的预后不良。
Hepatol Commun. 2022 May;6(5):1157-1171. doi: 10.1002/hep4.1866. Epub 2021 Nov 26.
2
CD90 glioma-associated mesenchymal stromal/stem cells promote temozolomide resistance by activating FOXS1-mediated epithelial-mesenchymal transition in glioma cells.CD90 阳性胶质母细胞瘤间质/干细胞通过激活 FOXS1 介导的上皮间质转化促进胶质母细胞瘤细胞对替莫唑胺耐药。
Stem Cell Res Ther. 2021 Jul 13;12(1):394. doi: 10.1186/s13287-021-02458-8.
3
FOXM1 and Cancer: Faulty Cellular Signaling Derails Homeostasis.
结直肠癌治疗的新视角：微小 RNA 的作用。
Curr Drug Targets. 2024;25(11):715-723. doi: 10.2174/0113894501304351240703113651.
4
Expression of CXCL8 and its relationship with prognosis in patients with non-small cell lung cancer.CXCL8在非小细胞肺癌患者中的表达及其与预后的关系。
Am J Cancer Res. 2024 Jun 15;14(6):2934-2945. doi: 10.62347/LJDQ3897. eCollection 2024.
5
Prediction of the survival status and tumor microenvironment in colorectal cancer through genotyping analysis based on toll-like receptors.基于 Toll 样受体的基因分型分析预测结直肠癌的生存状态和肿瘤微环境。
Saudi J Gastroenterol. 2024 Jul 1;30(4):243-251. doi: 10.4103/sjg.sjg_424_23. Epub 2024 May 30.
6
Comprehensive scRNA-seq Model Reveals Artery Endothelial Cell Heterogeneity and Metabolic Preference in Human Vascular Disease.全面的 scRNA-seq 模型揭示了人类血管疾病中动脉内皮细胞的异质性和代谢偏好。
Interdiscip Sci. 2024 Mar;16(1):104-122. doi: 10.1007/s12539-023-00591-x. Epub 2023 Nov 17.
7
Alterations in ileal transcriptomics during an intestinal barrier challenge in lactating Holstein cows fed a Saccharomyces cerevisiae fermentation product identify potential regulatory processes.在饲喂酿酒酵母发酵产物的泌乳荷斯坦奶牛的肠道屏障挑战期间，回肠转录组学的改变确定了潜在的调节过程。
J Anim Sci. 2023 Jan 3;101. doi: 10.1093/jas/skad277.
叉头框蛋白M1（FOXM1）与癌症：细胞信号传导异常破坏体内平衡。
Front Oncol. 2021 Feb 15;10:626836. doi: 10.3389/fonc.2020.626836. eCollection 2020.
4
Cancer Statistics, 2021.癌症统计数据，2021.
CA Cancer J Clin. 2021 Jan;71(1):7-33. doi: 10.3322/caac.21654. Epub 2021 Jan 12.
5
Forkhead Box S1 Inhibits the Progression of Hepatocellular Carcinoma.叉头框蛋白S1抑制肝细胞癌的进展。
Onco Targets Ther. 2020 Nov 17;13:11839-11848. doi: 10.2147/OTT.S272596. eCollection 2020.
6
CREB5 promotes invasiveness and metastasis in colorectal cancer by directly activating MET.CREB5 通过直接激活 MET 促进结直肠癌的侵袭和转移。
J Exp Clin Cancer Res. 2020 Aug 25;39(1):168. doi: 10.1186/s13046-020-01673-0.
7
Comparison of Different Colorectal Cancer With Liver Metastases Models Using Six Colorectal Cancer Cell Lines.比较六种结直肠癌细胞系建立的不同结直肠癌肝转移模型。
Pathol Oncol Res. 2020 Oct;26(4):2177-2183. doi: 10.1007/s12253-020-00805-3. Epub 2020 Mar 14.
8
Colorectal cancer statistics, 2020.2020 年结直肠癌统计数据。
CA Cancer J Clin. 2020 May;70(3):145-164. doi: 10.3322/caac.21601. Epub 2020 Mar 5.
9
FOXS1 is regulated by GLI1 and miR-125a-5p and promotes cell proliferation and EMT in gastric cancer.FOXS1 受 GLI1 和 miR-125a-5p 的调控，促进胃癌细胞的增殖和 EMT。
Sci Rep. 2019 Mar 27;9(1):5281. doi: 10.1038/s41598-019-41717-w.
10
KRAS-IRF2 Axis Drives Immune Suppression and Immune Therapy Resistance in Colorectal Cancer.KRAS-IRF2 轴驱动结直肠癌的免疫抑制和免疫治疗耐药性。
Cancer Cell. 2019 Apr 15;35(4):559-572.e7. doi: 10.1016/j.ccell.2019.02.008. Epub 2019 Mar 21.