Lee Sang-Nam, Park Ji-Ha, Ku Sae-Kwang
19Department of Anatomy and Histology, College of Oriental Medicine, Daegu Haany University, 290, Yugok-dong, Gyeongsan-si, Gyeongsangbuk-do, 712-715 Korea.
29Development Team for The New Drug of Oriental Medicine (BK21 program), Daegu Haany University, Gyeongsan, 712-715 Korea.
Toxicol Res. 2008 Sep;24(3):213-218. doi: 10.5487/TR.2008.24.3.213. Epub 2008 Sep 1.
In this research, the genotoxic effects of Kong-Jin-Dan (KJD), a polyherbal formula were evaluated using the mouse micronucleus test. KJD was administered once a day for 2 continuous days by oral gavage to male ICR mice at doses of 2000, 1000 and 500 mg/kg. Cyclophosphamide was used as a known genotoxic agent in a positive control. The appearance of a micronucleus is used as an index for genotoxic potential. In addition, the changes on the total white blood cells and differential counts on the lymphocytes, neutrophils, eosinophils, basophils and monocytes in the prepared blood smears were also conducted to observe the possible immunosuppress. The results obtained indicated that KJD shows no genotoxicity effects up to 2000 mg/kg dosing levels, but KJD shows slight increased trends in the blood total leukocyte numbers as pharmacological effects of immune stimulation. In addition, it is also considered that there were no problems from cytotoxicity of KJD tested in this study because the polychromatic erythrocyte ratio was detected as > 0.42 in all tested groups.
在本研究中，使用小鼠微核试验评估了复方中药制剂 Kong-Jin-Dan（KJD）的遗传毒性作用。将 KJD 以 2000、1000 和 500 mg/kg 的剂量通过口服灌胃连续 2 天每天一次给予雄性 ICR 小鼠。环磷酰胺用作阳性对照中的已知遗传毒性剂。微核的出现用作遗传毒性潜力的指标。此外，还对制备的血涂片进行了总白细胞计数以及淋巴细胞、中性粒细胞、嗜酸性粒细胞、嗜碱性粒细胞和单核细胞的分类计数变化，以观察可能的免疫抑制情况。所得结果表明，在高达 2000 mg/kg 的给药水平下，KJD 未显示出遗传毒性作用，但作为免疫刺激的药理作用，KJD 在血液总白细胞数量上显示出轻微增加的趋势。此外，由于在所有测试组中检测到多染红细胞比率>0.42，因此也认为本研究中测试的 KJD 的细胞毒性没有问题。
