Khan Mujeeb, Shaik Mohammed Rafi, Khan Shams Tabrez, Adil Syed Farooq, Kuniyil Mufsir, Khan Majad, Al-Warthan Abdulrahman A, Siddiqui Mohammed Rafiq H, Nawaz Tahir Muhammad
Department of Chemistry, College of Science, King Saud University, P.O. 2455, Riyadh 11451, Kingdom of Saudi Arabia.
Department of Agricultural Microbiology, Faculty of Agriculture, Aligarh Muslim University, Aligarh, Uttar Pradesh 202002, India.
ACS Omega. 2020 Jan 23;5(4):1987-1996. doi: 10.1021/acsomega.9b03840. eCollection 2020 Feb 4.
The effective interactions of nanomaterials with biological constituents play a significant role in enhancing their biomedicinal properties. These interactions can be efficiently enhanced by altering the surface properties of nanomaterials. In this study, we demonstrate the method of altering the surface properties of ZrO nanoparticles (NPs) to enhance their antimicrobial properties. To do this, the surfaces of the ZrO NPs prepared using a solvothermal method is functionalized with glutamic acid, which is an α-amino acid containing both COO and NH ions. The binding of glutamic acid (GA) on the surface of ZrO was confirmed by UV-visible and Fourier transform infrared spectroscopies, whereas the phase and morphology of resulting GA-functionalized ZrO (GA-ZrO) was identified by X-ray diffraction and transmission electron microscopy. GA stabilization has altered the surface charges of the ZrO, which enhanced the dispersion qualities of NPs in aqueous media. The as-prepared GA-ZrO NPs were evaluated for their antibacterial properties toward four strains of oral bacteria, namely, , , , and . GA-ZrO exhibited increased antimicrobial activities compared with pristine ZrO. This improved activity can be attributed to the alteration of surface charges of ZrO with GA. Consequently, the dispersion properties of GA-ZrO in the aqueous solution have increased considerably, which may have enhanced the interactions between the nanomaterial and bacteria.
纳米材料与生物成分之间的有效相互作用在增强其生物医学特性方面发挥着重要作用。通过改变纳米材料的表面性质，可以有效地增强这些相互作用。在本研究中，我们展示了改变ZrO纳米颗粒（NPs）表面性质以增强其抗菌性能的方法。为此，使用溶剂热法制备的ZrO NPs的表面用谷氨酸进行功能化，谷氨酸是一种同时含有COO和NH离子的α-氨基酸。通过紫外可见光谱和傅里叶变换红外光谱证实了谷氨酸（GA）在ZrO表面的结合，而通过X射线衍射和透射电子显微镜确定了所得GA功能化ZrO（GA-ZrO）的相和形态。GA稳定化改变了ZrO的表面电荷，增强了NPs在水性介质中的分散质量。对制备的GA-ZrO NPs对四种口腔细菌菌株，即 、 、 和 的抗菌性能进行了评估。与原始ZrO相比，GA-ZrO表现出增强的抗菌活性。这种活性的提高可归因于GA对ZrO表面电荷的改变。因此，GA-ZrO在水溶液中的分散性能显著提高，这可能增强了纳米材料与细菌之间的相互作用。
