Department of Bioinformatics, Smart Health Big Data Analysis and Location Services Engineering Lab of Jiangsu Province, School of Geographic and Biologic Information, Nanjing University of Posts and Telecommunications, Nanjing 210023, China.
Hepatobiliary Center, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
Aging (Albany NY). 2020 Feb 10;12(3):2626-2646. doi: 10.18632/aging.102766.
Cholangiocarcinoma (CCA), an aggressive tumor with poor prognosis, is a malignant cancer with increasing incidence and mortality rates. It is important to survey crucial genes in CCA to find and design potential drug targets, especially for those genes associated with cell proliferation that is a key biological process in tumorgenesis. Herein, we surveyed genes associated with cell proliferation via a comprehensive pan-cancer analysis. Candidate genes were further analyzed using multiple approaches, including cross-analysis from diverse molecular levels, examination of potential function and interactions, and additional experimental validation. We primarily screened 15 potential genes based on 11 validated genes, and these 26 genes were further examined to delineate their biological functions and potential roles in cancer treatment. Several of them were involved synthetically lethal genetic interactions, especially for , , and , indicating their potential roles in drug design and cancer treatment. Further experimental validation indicated that some genes were significantly upregulated in several cancer cell lines, implying their important roles in tumorigenesis. Our study identifies some genes associated with cell proliferation, which may be potential future targets in molecular targeted therapy.
胆管癌（CCA）是一种预后不良的侵袭性肿瘤，其发病率和死亡率呈上升趋势。调查 CCA 中的关键基因对于寻找和设计潜在的药物靶点非常重要，特别是那些与细胞增殖相关的基因，因为细胞增殖是肿瘤发生的关键生物学过程。在这里，我们通过全面的泛癌症分析调查了与细胞增殖相关的基因。候选基因进一步通过多种方法进行分析，包括来自不同分子水平的交叉分析、对潜在功能和相互作用的检查以及额外的实验验证。我们主要基于 11 个已验证的基因筛选了 15 个潜在基因，然后进一步检查了这 26 个基因，以阐明它们在癌症治疗中的生物学功能和潜在作用。其中一些基因参与了综合致死性遗传相互作用，特别是对于、、和，表明它们在药物设计和癌症治疗中有潜在作用。进一步的实验验证表明，一些基因在几种癌细胞系中显著上调，暗示它们在肿瘤发生中具有重要作用。我们的研究确定了一些与细胞增殖相关的基因，这些基因可能是未来分子靶向治疗的潜在靶点。
