Asahi Yoh, Hatanaka Kanako C, Hatanaka Yutaka, Kamiyama Toshiya, Orimo Tatsuya, Shimada Shingo, Nagatsu Akihisa, Sakamoto Yuzuru, Kamachi Hirofumi, Kobayashi Nozomi, Fukai Moto, Taketomi Akinobu
Department of Gastroenterological Surgery I, Hokkaido University Graduate School of Medicine, Kita-ku, Kita 15, Nishi 7, Sapporo, Hokkaido, 060-8638, Japan.
Clinical Biobank, Clinical Research and Medical Innovation Center, Hokkaido University Hospital, Kita-ku, Kita 15, Nishi 7, Sapporo, Hokkaido, 060-8638, Japan.
Surg Today. 2020 Aug;50(8):931-940. doi: 10.1007/s00595-020-01967-y. Epub 2020 Feb 10.
A lack of effective systemic therapy is one reason for the poor prognosis of intrahepatic cholangiocarcinoma. Newly developed immune checkpoint inhibitors function by minimizing CD8+ T cell suppression to improve tumor-specific responses. This study aimed to examine the characteristics of CD8+ T cells in intrahepatic cholangiocarcinoma.
Clinicopathological data, including the overall survival, of 69 cases of postoperative intrahepatic cholangiocarcinoma were prospectively investigated. We then immunohistochemically stained for CD8, Foxp3, CD163, PD-L1, and human leukocyte antigen (HLA) class I and counted the number of CD8+ T cells, Foxp3+ T cells, and CD163+ macrophages in different areas (outer border, interborder, and intratumor).
A significant difference was found in the 5-year overall survival between the CD8+ T cell high group (45.5%) and low group (24.7%) in the outer border area (p = 0.0103). Furthermore, the number of CD8+ T cells and the high expression of HLA class I were positively correlated (p = 0.0341).
The number of CD8+ T cells in the outer border area of the tumor correlated with the HLA class I expression of intrahepatic cholangiocarcinoma and may therefore be a prognostic factor for patients with postoperative intrahepatic cholangiocarcinoma.
缺乏有效的全身治疗是肝内胆管癌预后不良的原因之一。新开发的免疫检查点抑制剂通过最小化CD8 + T细胞抑制来发挥作用，以改善肿瘤特异性反应。本研究旨在探讨肝内胆管癌中CD8 + T细胞的特征。
前瞻性调查69例肝内胆管癌术后患者的临床病理数据，包括总生存期。然后我们对CD8、Foxp3、CD163、PD-L1和人类白细胞抗原（HLA）I类进行免疫组化染色，并计数不同区域（外边界、边界和肿瘤内）的CD8 + T细胞、Foxp3 + T细胞和CD163 +巨噬细胞数量。
在外边界区域，CD8 + T细胞高表达组（45.5%）和低表达组（24.7%）的5年总生存期存在显著差异（p = 0.0103）。此外，CD8 + T细胞数量与HLA I类的高表达呈正相关（p = 0.0341）。
肿瘤外边界区域的CD8 + T细胞数量与肝内胆管癌的HLA I类表达相关，因此可能是肝内胆管癌术后患者的一个预后因素。
