State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, 712100, Shaanxi, China.
Key Laboratory of Green Prevention and Control of Tropical Plant Disease and Pests, Ministry of Education and College of Plant Protection, Hainan University, Haikou, 570228, Hainan, China.
Arch Virol. 2020 Apr;165(4):1011-1014. doi: 10.1007/s00705-020-04545-2. Epub 2020 Feb 10.
The complete genome of a novel fungal virus, Puccinia striiformis narnavirus 1 (PsNV1), was sequenced and analyzed. The full-length cDNA sequence is 2340 bp in length with a GC content of 50.04%. PsNV1 contains a single open reading frame (ORF), which encodes a putative RNA-dependent RNA polymerase (RdRp) of 741 amino acids with a molecular mass of 81.8 kDa. RdRp phylogeny showed that PsNV1 grouped together with Fusarium poae narnavirus 1 (FpNV1) as a sister branch of narnaviruses, forming a distinct clade. The results of genome sequence comparisons and phylogenetic analysis indicate that PsNV1 is a new member in the genus Narnavirus. To our knowledge, this is the first report of a narnavirus genome sequence in the obligately parasitic fungus Puccinia striiformis f. sp. tritici.
我们对一种新型真菌病毒——条锈菌冬孢菌病毒 1（PsNV1）的完整基因组进行了测序和分析。其全长 cDNA 序列为 2340 个碱基对，GC 含量为 50.04%。PsNV1 包含一个单一的开放阅读框（ORF），编码一个推定的 RNA 依赖性 RNA 聚合酶（RdRp），由 741 个氨基酸组成，分子量为 81.8 kDa。RdRp 系统发育分析表明，PsNV1 与禾旋孢腔菌冬孢菌病毒 1（FpNV1）聚为一簇，与纳罗病毒为姐妹分支，形成一个独特的进化枝。基因组序列比较和系统发育分析结果表明，PsNV1 是纳罗病毒属的一个新成员。据我们所知，这是首次在专性寄生真菌条锈菌冬孢菌 f. sp. tritici 中报道纳罗病毒基因组序列。
