在小麦叶锈病菌中鉴定出的一种新型线粒体病毒的全基因组序列
Complete genome sequence of a novel mitovirus identified in the phytopathogenic fungus Puccinia triticina.
作者信息
Zhang Yanhui, Wang Nuoheng, Li Jinyang, Chen Bingtao, Kang Zhensheng, Song Pengyu, Zheng Wenming
机构信息
State Key Laboratory of Wheat and Maize Crop Science, College of Life Sciences, Henan Agricultural University, Zhengzhou, Henan, 450046, China.
State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, Shaanxi, 712100, China.
出版信息
Arch Virol. 2025 Mar 27;170(5):90. doi: 10.1007/s00705-025-06272-y.
Wheat leaf rust is caused by the obligate biotrophic fungus Puccinia triticina f. sp. tritici, which seriously affects wheat production. In this study, a novel mitovirus was identified in Puccinia triticina strain HN-1 and designated as "Puccinia triticina mitovirus 1" (PtMV1). The genome of PtMV1 consists of a single RNA molecule with a length of 2,380 nt and an A + U content of 54.7% that contains a single open reading frame (ORF). The ORF is predicted to encode a putative RNA-dependent RNA polymerase (RdRp) of 653 amino acids with a molecular mass of 74.77 kDa, containing six conserved motifs. The RdRp amino acid sequence of PtMV1 has a high degree of sequence similarity to the RdRps of unuamitoviruses. Phylogenetic analysis indicated that PtMV1 is a new member of the genus Unuamitovirus within the family Mitoviridae. To our knowledge, this is the first report of a fungal virus in Puccinia triticina.
小麦叶锈病由专性活体营养真菌小麦柄锈菌小麦专化型（Puccinia triticina f. sp. tritici）引起，严重影响小麦生产。在本研究中，从小麦柄锈菌HN-1菌株中鉴定出一种新型的线粒体病毒，并命名为“小麦柄锈菌线粒体病毒1”（PtMV1）。PtMV1的基因组由一个长度为2380 nt的单链RNA分子组成，A+U含量为54.7%，包含一个单一的开放阅读框（ORF）。该ORF预计编码一个由653个氨基酸组成的推定RNA依赖RNA聚合酶（RdRp），分子量为74.77 kDa，包含六个保守基序。PtMV1的RdRp氨基酸序列与未分类线粒体病毒的RdRp具有高度的序列相似性。系统发育分析表明，PtMV1是线粒体病毒科未分类线粒体病毒属的一个新成员。据我们所知，这是关于小麦柄锈菌中真菌病毒的首次报道。
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