State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling 712100, China.
Hainan Yazhou Bay Seed Laboratory, Sanya Nanfan Research Institute of Hainan University, Sanya 572025, China.
Viruses. 2023 May 28;15(6):1265. doi: 10.3390/v15061265.
Wheat stripe rust, caused by the obligate biotrophic fungus f. sp. (), seriously affects wheat production. Here, we report the complete genome sequence and biological characterization of a new mitovirus from strain GS-1, which was designated as "Puccinia striiformis mitovirus 2" (PsMV2). Genome sequence analysis showed that PsMV2 is 2658 nt in length with an AU-rich of 52.3% and comprises a single ORF of 2348 nt encoding an RNA-dependent RNA polymerase (RdRp). Phylogenetic analysis indicated that PsMV2 is a new member of the genus within the family In addition, PsMV2 multiplied highly during infection and it suppresses programmed cell death (PCD) triggered by Bax. Silencing of PsMV2 in by barley stripe mosaic virus (BSMV)-mediated Host Induced Gene Silencing (HIGS) reduced fungal growth and decreased pathogenicity of . These results indicate PsMV2 promotes host pathogenicity in . Interestingly, PsMV2 was detected among a wide range of field isolates of and may have coevolved with in earlier times. Taken together, our results characterized a novel mitovirus PsMV2 in wheat stripe rust fungus, which promotes the virulence of its fungal host and wide distribution in which may offer new strategies for disease control.
小麦条锈病由专性活体营养真菌 f. sp. 引起()，严重影响小麦生产。在这里，我们报告了一种来自 菌株 GS-1 的新的呼肠孤病毒的完整基因组序列和生物学特征，该病毒被命名为“条锈菌呼肠孤病毒 2”（PsMV2）。基因组序列分析表明，PsMV2 长 2658nt，AU 含量为 52.3%，包含一个编码 RNA 依赖性 RNA 聚合酶（RdRp）的 2348nt 的单一 ORF。系统发育分析表明，PsMV2 是呼肠孤病毒科属的一个新成员。此外，PsMV2 在 感染过程中高度增殖，并抑制 Bax 触发的程序性细胞死亡（PCD）。通过大麦黄花叶病毒（BSMV）介导的寄主诱导基因沉默（HIGS）沉默 中的 PsMV2 ，降低了真菌的生长并降低了 的致病性。这些结果表明 PsMV2 在 中促进了宿主的致病性。有趣的是，PsMV2 在广泛的小麦条锈菌田间分离物中被检测到，并且可能在早期与 共同进化。总之，我们的结果描述了小麦条锈菌中的一种新型呼肠孤病毒 PsMV2，它促进了其真菌宿主的毒力和在 中的广泛分布，这可能为疾病控制提供了新的策略。
