Alfaki Shima H, Hussien Mohammed O, Osman Nussieba A, Enan Khalid A, El Hussein Abdel Rahim M
Central Veterinary Research Laboratory (CVRL), Animal Resources Research Corporation (ARRC), P.O. Box 8067, El Amarat, Khartoum, Sudan.
Central Laboratory, Ministry of Higher Education and Scientific Research, P.O. Box 7099, Khartoum, Sudan.
Trop Anim Health Prod. 2020 Jul;52(4):2073-2078. doi: 10.1007/s11250-020-02235-4. Epub 2020 Feb 10.
The reticuloendotheliosis virus (REV) group of retroviruses infects a wide range of avian species, including chickens, turkeys, ducks, geese, quail, and prairie chickens. The infection can result in immunosuppression, runting syndrome, high mortality, acute reticular cell neoplasia, or T- and/or B-cell lymphoma. One PCR positive chicken spleen sample obtained in a previous study in addition to one Marek's disease and three fowl pox (FP) vaccine samples were investigated in this study. A PCR assay was performed to detect the presence of REV provirus DNA in these samples. The results indicated the contamination of fowl pox virus and Marek's disease vaccines with REV. In addition, detection of integration of REV inside the genome of fowl pox vaccine was confirmed using primers corresponding to the FPV DNA regions flanking the REV integration site. Alignments of two sequences, one from the spleen tissue and the other from contaminated FP vaccine with REV, with other REV (env) gene sequences obtained from GenBank indicated their high similarity. Furthermore, phylogenetic analysis indicated that the partial part of (env) gene of our two isolates was closely related to variants from India, USA, Taiwan, and China. These results confirmed the contamination of commercial fowl pox and Marek's disease vaccines used in Sudan with REV. Phylogenetic analysis indicated that the partial part of (env) gene sequences from Sudan was closely related to variants from India, USA, Taiwan, and China.
逆转录病毒网状内皮增生症病毒（REV）组可感染多种禽类，包括鸡、火鸡、鸭、鹅、鹌鹑和草原鸡。这种感染可导致免疫抑制、生长迟缓综合征、高死亡率、急性网状细胞瘤或T细胞和/或B细胞淋巴瘤。本研究对先前一项研究中获得的一份PCR阳性鸡脾脏样本以及一份马立克氏病疫苗样本和三份鸡痘（FP）疫苗样本进行了调查。采用PCR检测这些样本中REV前病毒DNA的存在情况。结果表明鸡痘病毒和马立克氏病疫苗被REV污染。此外，使用与REV整合位点侧翼的FPV DNA区域对应的引物，证实了REV在鸡痘疫苗基因组内的整合检测。将来自脾脏组织的一个序列以及来自被REV污染的FP疫苗的另一个序列与从GenBank获得的其他REV（env）基因序列进行比对，结果表明它们具有高度相似性。此外，系统发育分析表明，我们的两个分离株的（env）基因部分与来自印度、美国、台湾和中国的变体密切相关。这些结果证实了苏丹使用的商业鸡痘疫苗和马立克氏病疫苗被REV污染。系统发育分析表明，来自苏丹的（env）基因序列部分与来自印度、美国、台湾和中国的变体密切相关。
