泰国鸡的网状内皮组织增生病病毒的遗传特征。

Genetic characterization of reticuloendotheliosis virus in chickens in Thailand.

机构信息

Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.

Center of Excellence for Emerging and Re-emerging Infectious Diseases in Animals (CUEIDAs), Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.

出版信息

Poult Sci. 2019 Jun 1;98(6):2432-2438. doi: 10.3382/ps/pez025.

DOI:10.3382/ps/pez025

PMID:30668827

Abstract

Reticuloendotheliosis virus (REV) causes an immunosuppressive, runting, and oncogenic disease in poultry, posing a significant threat to the poultry industry. In Thailand, an unidentified disease associated with runting-stunting syndrome and neoplasia, resembling REV infection, has been continuously observed in several chicken farms. However, REV infection in Thailand has never been reported. In this study, we investigated the occurrence and genetic characteristics of REVs in chickens in Thailand from 2013 to 2016. Of the 130 clinical samples obtained from 29 chicken farms from 9 provinces located in the major chicken-raising regions of Thailand, including the central, eastern, northern, and northeastern parts of Thailand, 51 samples (39.23%) and 21 farms (72.41%) were REV-positive. REV-positive samples were detected in all 9 provinces tested. Our results demonstrated that REV was extensively distributed in the major chicken-raising regions of Thailand. Phylogenetic analysis of the whole genome sequence showed that Thai REV was most closely related to Chinese, Taiwanese, and the US REV strains isolated from different avian species and clustered into REV subtype III. This finding indicates that REV subtype III was predominantly circulated in Thai chicken flocks. This study is the first report on REV infection in chickens in Thailand. Our findings raise the awareness of REV as another causative agent of runting and oncogenic disease in chickens in Thailand and highlight the wide distribution of REV infection among chickens worldwide.

摘要

网状内皮组织增生症病毒（REV）可引起禽类免疫抑制、生长迟缓及致癌疾病，对家禽业构成重大威胁。在泰国，一种与生长迟缓综合征和肿瘤形成相关、类似于 REV 感染的不明疾病，已在多个鸡场持续观察到。然而，泰国从未报告过 REV 感染。本研究于 2013 年至 2016 年期间，调查了泰国鸡群中 REV 的发生情况及其遗传特征。从泰国主要养鸡地区（包括中部、东部、北部和东北部）的 9 个省的 29 个鸡场采集了 130 份临床样本，其中 51 份（39.23%）和 21 个鸡场（72.41%）为 REV 阳性。9 个检测省份均检测到 REV 阳性样本。研究结果表明，REV 在泰国主要养鸡地区广泛分布。全基因组序列的系统进化分析表明，泰国 REV 与中国、中国台湾和美国从不同禽种分离的 REV 株最为密切相关，聚类为 REV 亚 III 型。这一发现表明，REV 亚 III 型主要在泰国鸡群中流行。本研究首次报道了泰国鸡群中 REV 感染。我们的研究结果表明，REV 是泰国鸡群中另一种引起生长迟缓和致癌疾病的病原体，并强调了 REV 感染在全球鸡群中的广泛分布。