Ren Hong, Zhou Ping, Shen Xiaoyun
North Sichuan Medical College, Nanchong 637100, China.
State Key Laboratory of Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi 832000, China.
Metabolites. 2023 Aug 30;13(9):982. doi: 10.3390/metabo13090982.
Selenium (Se)-enriched habitats have led to chronic selenosis, seriously affecting the health and survival of Procapra przewalskii (. ). Our targets were to explore the molecular mechanisms of chronic selenosis and to look for a new way to protect endangered species. The mineral contents of soils, grass, blood, and muscle were analyzed. The biochemical indices, antioxidant capability, and immune function were also investigated. The analyses of proteomics and metabolomics were also carried out. The results showed that the Se contents in the muscle and blood of . , and the soil and grass in the Se-enriched habitats were significantly higher than those in healthy pastures. The . in the Se-enriched habitats showed symptoms of anemia, decreased antioxidant capability, and low immune function. A total of 44 differential proteins and 36 differential metabolites were screened by analyzing their proteomics and metabolomics. These differential proteins and metabolites were involved in glycolysis pathway, amino acid biosynthesis, carbon metabolism, phenylalanine metabolism, and energy metabolism. In particular, phenylalanine metabolism was the common pathway of proteomics and metabolomics, which was an important finding in studying the mechanism of chronic selenosis in animals. This study will help us to further understand the mechanism of chronic selenosis in . , and it provides a scientific basis for the protection of endangered species in Se-enriched habitats.
富硒栖息地导致了慢性硒中毒，严重影响了普氏原羚的健康和生存。我们的目标是探索慢性硒中毒的分子机制，并寻找保护濒危物种的新方法。分析了土壤、牧草、血液和肌肉中的矿物质含量。还研究了生化指标、抗氧化能力和免疫功能。进行了蛋白质组学和代谢组学分析。结果表明，普氏原羚肌肉和血液中的硒含量以及富硒栖息地的土壤和牧草中的硒含量显著高于健康牧场。富硒栖息地的普氏原羚出现贫血症状、抗氧化能力下降和免疫功能低下。通过分析其蛋白质组学和代谢组学，共筛选出44种差异蛋白和36种差异代谢物。这些差异蛋白和代谢物参与糖酵解途径、氨基酸生物合成、碳代谢、苯丙氨酸代谢和能量代谢。特别是，苯丙氨酸代谢是蛋白质组学和代谢组学的共同途径，这是研究动物慢性硒中毒机制的一个重要发现。本研究将有助于我们进一步了解普氏原羚慢性硒中毒的机制，并为富硒栖息地濒危物种的保护提供科学依据。
