Taubert G, Krug H
Department of Quantitative Morphology, Karl Marx University Leipzig, German Democratic Republic.
J Cancer Res Clin Oncol. 1988;114(6):559-64. doi: 10.1007/BF00398177.
DNA histograms of human tumors should be interpreted together with the type of growth. In order to prove this connection the Ehrlich ascites tumors of 144 mice were investigated by absolute cell counting and flow cytometry. The exponential, transition and steady-state phases of tumor development were defined as types of growth based on the logistic function (Verhulst-Pearl). Flow-cytometric histograms (ethidium-bromide, olivomycin) were evaluated by two methods presupposing lognormal distribution and resulting in the same trend of changes concerning the percentage of cells in the three cell-cycle phases. In exponential growth the histograms show a high S-phase compartment and a decrease of the G2M peak. The increased percentage of S-phase cells is caused by a recruitment from G0-1 and G0-2 after transplantation. The decrease of the G2M peak is due to the exponential growth, a recruitment from G0-2 and a relative shortening of G2M duration. A high G2M peak and a low S-phase portion occur in steady state. Besides a decrease of DNA-synthesizing cells the S compartment decreases as a result of a relative S-phase shortening. For the increase of the G2M peak, transition into steady state, prolongation of G2M duration, recruitment of G0-1 and the occurrence of G0-2 cells are responsible. The same histogram may reflect different biological behavior patterns depending on the type of growth.
人类肿瘤的DNA直方图应结合生长类型进行解读。为了证明这种关联，通过绝对细胞计数和流式细胞术对144只小鼠的艾氏腹水瘤进行了研究。基于逻辑斯蒂函数（Verhulst-Pearl）将肿瘤发展的指数期、过渡期和稳态期定义为生长类型。通过两种假定对数正态分布的方法对流式细胞术直方图（溴化乙锭、橄榄霉素）进行评估，结果显示三个细胞周期阶段的细胞百分比变化趋势相同。在指数生长阶段，直方图显示S期细胞比例高，G2M峰降低。S期细胞百分比增加是由于移植后从G0-1期和G0-2期募集细胞所致。G2M峰降低是由于指数生长、从G0-2期募集细胞以及G2M持续时间相对缩短。在稳态期出现高G2M峰和低S期比例。除了DNA合成细胞减少外，S期部分也因S期相对缩短而减少。G2M峰增加、转变为稳态、G2M持续时间延长、从G0-1期募集细胞以及G0-2期细胞出现是导致这种情况的原因。根据生长类型，相同的直方图可能反映不同的生物学行为模式。
