Krug H, Schönfelder M
Acta Histochem Suppl. 1984;30:117-27.
CEll suspensions from 62 cancers of breast were investigated by flow cytophotometry after staining with pepsin-Ethidium bromide. In all patients a radical operation was made, followed by radiotherapy. The surviving patients were observed for 5 years. From the DNA-histograms 48% of the tumours were classified as diploid (or peridiploid), 40% as polyploid and 11% as aneuploid. Concerning ploidy a rank with worsening prognosis was found from diploid over polyploid to aneuploid cancers. In this sense bad prognosis means: dead or relapse during 5 years, histologically undifferentiated tumours, great primary tumour, metastasis in lymph nodes. In diploid tumours bad prognosis is indicated by a higher part of S- and G2-phase nuclei in the flow histograms. Most of the findings, but not all, are proved statistically. Now as ever, histological differentiation is still the most easily statement for prognosis.
用胃蛋白酶-溴化乙锭染色后，通过流式细胞光度术对62例乳腺癌的细胞悬液进行了研究。所有患者均接受了根治性手术，随后进行放疗。对存活患者进行了5年观察。从DNA直方图来看，48%的肿瘤被分类为二倍体（或近二倍体），40%为多倍体，11%为非整倍体。关于倍性，发现从二倍体癌到多倍体癌再到非整倍体癌，预后逐渐恶化。从这个意义上说，预后不良意味着：5年内死亡或复发、组织学上未分化的肿瘤、原发肿瘤较大、淋巴结转移。在二倍体肿瘤中，流式直方图中S期和G2期细胞核比例较高表明预后不良。大多数研究结果，但并非全部，都得到了统计学验证。一如既往，组织学分化仍然是最容易判断预后的指标。
