Department of Histology and Cell Biology, Benha Faculty of Medicine, Benha University, Benha, Egypt.
Ultrastruct Pathol. 2020 Mar 3;44(2):159-173. doi: 10.1080/01913123.2020.1726547. Epub 2020 Feb 10.
Lipopolysaccharide (LPS) is an endotoxin that prompts neuroinflammation and initiates neurodegenerative disorders. Exosome is a recent therapeutic agent for many diseases such as neurological diseases. This study aimed to evaluate the potential protective role of bone marrow mesenchymal stem cell-derived exosomes (BMSC-EXs) in cerebellar cortex LPS-induced neuroinflammation in rats. Twenty-seven adult male rats were divided into three groups: Group I: control rats; Group II: LPS-treated rats; Group III: LPS/BMSC-EXs-treated rats. Cerebellar specimens were taken and processed for histological and immunohistochemical analysis. Morphometrical studies and statistical analysis were done. Groups II showed neuronal degeneration and apoptosis. The mean number of Purkinje cells was significantly (P<0.01) decreased, while glial fibrillary acidic protein (GFAP) immunoexpression was significantly increased in the neuroglial cells. Ultrastructural examination showed shrunken Purkinje cells with irregular nuclei and disrupted mitochondria. Group III showed improvement of most of the changes mentioned previously. EXs therapy is a promising neuroprotective tool for treatment of LPS-induced neuroinflammation.
脂多糖（LPS）是一种内毒素，能引发神经炎症，引发神经退行性疾病。外泌体是一种治疗多种疾病的新型治疗药物，如神经疾病。本研究旨在评估骨髓间充质干细胞来源的外泌体（BMSC-EXs）在大鼠小脑皮质 LPS 诱导的神经炎症中的潜在保护作用。27 只成年雄性大鼠分为三组：I 组：对照组；II 组：LPS 处理组；III 组：LPS/BMSC-EXs 处理组。取小脑标本进行组织学和免疫组织化学分析。进行形态计量学研究和统计分析。II 组显示神经元变性和凋亡。浦肯野细胞的平均数量显著减少（P<0.01），而神经胶质细胞中的胶质纤维酸性蛋白（GFAP）免疫表达显著增加。超微结构检查显示浦肯野细胞皱缩，核不规则，线粒体破裂。III 组显示出上述大多数变化的改善。EXs 治疗是一种有前途的神经保护工具，可用于治疗 LPS 诱导的神经炎症。
Zotero 插件