Linc01234 通过 miR-433/PAK4 轴促进口腔鳞状细胞癌的细胞增殖和转移。

Linc01234 promotes cell proliferation and metastasis in oral squamous cell carcinoma via miR-433/PAK4 axis.

机构信息

Department of Oral and Maxillofacial Surgery, Affiliated Haikou Hospital, Xiangya Medical College, Central South University, Haikou, 570208, China.

Department of Oral and Maxillofacial Surgery, Xiangya Hospital, Central South University, Changsha, 410008, China.

出版信息

BMC Cancer. 2020 Feb 10;20(1):107. doi: 10.1186/s12885-020-6541-0.

DOI:10.1186/s12885-020-6541-0

PMID:32041570

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7011552/

Abstract

BACKGROUND

Increasing studies have demonstrated that long non-coding RNAs (lncRNAs) play an important role in tumor progression. However, the potential biological functions and clinical importance of Linc01234 in oral squamous cell carcinoma (OSCC) remain unclear.

METHODS

We evaluated the expression profile and prognostic value of Linc01234 in OSCC tissues by RT-qPCR. Then, functional in vitro experiments were performed to investigate the effects of Linc01234 on tumor growth, migration and invasion in OSCC. Mechanistically, RT-qPCR, bioinformatic analysis and dual luciferase reporter assays were performed to identify a competitive endogenous RNA (ceRNA) mechanism involving Linc01234, miR-433-3p and PAK4.

RESULTS

We found that Linc01234 was clearly upregulated in OSCC tissues and cell lines, and its level was positively associated with T stage, lymph node metastasis, differentiation and poor prognosis of patients with OSCC. Our results shown that Linc01234 inhibited cell proliferation and metastatic abilities in CAL27 and SCC25 cells following its knockdown. Mechanistic analysis indicated that Linc01234 may act as a ceRNA (competing endogenous RNA) of miR-433-3p to relieve the repressive effect of miR-433-3p on its target PAK4.

CONCLUSIONS

Our results indicated that Linc01234 promotes OSCC progression through the Linc01234/miR-433/PAK4 axis and might be a potential therapeutic target for OSCC.

摘要

背景

越来越多的研究表明，长链非编码 RNA（lncRNA）在肿瘤进展中发挥重要作用。然而，Linc01234 在口腔鳞状细胞癌（OSCC）中的潜在生物学功能和临床重要性仍不清楚。

方法

我们通过 RT-qPCR 评估了 Linc01234 在 OSCC 组织中的表达谱和预后价值。然后，进行了功能体外实验，以研究 Linc01234 对 OSCC 中肿瘤生长、迁移和侵袭的影响。通过 RT-qPCR、生物信息学分析和双荧光素酶报告基因实验，确定了涉及 Linc01234、miR-433-3p 和 PAK4 的竞争性内源 RNA（ceRNA）机制。

结果

我们发现 Linc01234 在 OSCC 组织和细胞系中明显上调，其水平与 T 分期、淋巴结转移、分化和 OSCC 患者的预后呈正相关。我们的结果表明，Linc01234 下调后可抑制 CAL27 和 SCC25 细胞的增殖和转移能力。机制分析表明，Linc01234 可能作为 miR-433-3p 的 ceRNA（竞争性内源 RNA），减轻 miR-433-3p 对其靶标 PAK4 的抑制作用。