Department of Public Health and Infectious Diseases, Section of Parasitology, Sapienza-University of Rome, P.le Aldo Moro 5, 00185 10 Rome, Italy - Department of Ecological and Biological Sciences, Tuscia University, Viale dell'Università s/n, 01100 Viterbo, Italy.
Department of Ecological and Biological Sciences, Tuscia University, Viale dell'Università s/n, 01100 Viterbo, Italy.
Parasite. 2020;27:9. doi: 10.1051/parasite/2020004. Epub 2020 Feb 11.
Eight microsatellite loci, recently developed in the species Anisakis pegreffii, were successfully amplified in Anisakis berlandi, sibling species of the A. simplex (s. l.) complex. They were validated on adult specimens (n = 46) of the parasite species, collected from two individuals of the definitive host, the long-finned pilot whale Globicephala melas from New Zealand waters. Among the eight loci scored, one, Anisl 07132, had null alleles in A. berlandi and was thus excluded from the subsequent genetic analysis. Two loci, Anisl 00314 and Anisl 10535, were monomorphic. In addition, as also previously detected in the other species of the A. simplex (s. l.) complex, the Anisl 7 locus was seen to be sex-linked, showing hemizygosity in male specimens. Differential allele frequency distributions of A. berlandi, with respect to those previously observed in A. pegreffii and A. simplex (s. s.), were found at some microsatellite loci. The Anisl 7 locus provided 100% diagnosis between A. berlandi and A. pegreffii, while others resulted in 99% diagnosis between A. berlandi and the other two species. Simple sequence repeat (SSR) loci also allowed us to estimate the genetic differentiation of A. berlandi from A. pegreffii (F ≈ 0.45, Dc = 0.82) and A. simplex (s. s.) (F ≈ 0.57, Dc = 0.73). The results suggest that SSRs provide a set of candidate markers for population genetics analysis of A. berlandi, as well as for the investigation, through a multi-locus genotyping approach, of possible patterns of hybridisation/introgression events between A. berlandi and the other two Anisakis species in sympatric conditions.
八个微卫星基因座，最近在棘头虫属 Pegreffi 物种中开发，成功扩增在棘头虫属伯氏虫，棘头虫属 Simplex (s. l.) 复合体的姊妹种。它们在寄生虫物种的成年标本（n = 46）上得到验证，这些标本来自新西兰水域长鳍领航鲸的两个确定宿主个体。在所评分的 8 个基因座中，一个基因座 Anisl 07132 在棘头虫属伯氏虫中存在无效等位基因，因此被排除在随后的遗传分析之外。两个基因座 Anisl 00314 和 Anisl 10535 是单态的。此外，正如在棘头虫属 Simplex (s. l.) 复合体的其他物种中也发现的那样，Anisl 7 基因座被发现是性连锁的，在雄性标本中表现出半合子性。在一些微卫星基因座上，发现了棘头虫属伯氏虫相对于棘头虫属 Pegreffi 和棘头虫属 Simplex (s. s.) 先前观察到的差异等位基因频率分布。Anisl 7 基因座提供了棘头虫属伯氏虫和棘头虫属 Pegreffi 之间 100%的诊断，而其他基因座则在棘头虫属伯氏虫和其他两个物种之间提供了 99%的诊断。简单重复序列（SSR）基因座也使我们能够估计棘头虫属伯氏虫与棘头虫属 Pegreffi（F ≈ 0.45，Dc = 0.82）和棘头虫属 Simplex (s. s.)（F ≈ 0.57，Dc = 0.73）之间的遗传分化。结果表明，SSR 为棘头虫属伯氏虫的种群遗传学分析提供了一组候选标记，也为通过多基因座基因分型方法研究棘头虫属伯氏虫与其他两种棘头虫属物种在同域条件下可能发生的杂交/基因渗透事件的模式提供了可能。
