UCIBIO, Chemistry Department, School of Sciences and Technology, NOVA University of Lisbon, 2829-516 Caparica, Portugal.
UCIBIO, Chemistry Department, School of Sciences and Technology, NOVA University of Lisbon, 2829-516 Caparica, Portugal.
J Chromatogr A. 2020 May 24;1619:460871. doi: 10.1016/j.chroma.2020.460871. Epub 2020 Jan 11.
Affinity adsorbents have been the cornerstone in protein purification. The selective nature of the molecular recognition interactions established between an affinity ligands and its target provide the basis for efficient capture and isolation of proteins. The plethora of affinity adsorbents available in the market reflects the importance of affinity chromatography in the bioseparation industry. Ligand discovery relies on the implementation of rational design techniques, which provides the foundation for the engineering of novel affinity ligands. The main goal for the design of affinity ligands is to discover or improve functionality, such as increased stability or selectivity. However, the methodologies must adapt to the current needs, namely to the number and diversity of biologicals being developed, and the availability of new tools for big data analysis and artificial intelligence. In this review, we offer an overview on the development of affinity ligands for bioseparation, including the evolution of rational design techniques, dating back to the years of early discovery up to the current and future trends in the field.
亲和吸附剂一直是蛋白质纯化的基石。亲和配体与目标之间建立的分子识别相互作用的选择性为蛋白质的有效捕获和分离提供了基础。市场上有大量的亲和吸附剂，这反映了亲和层析在生物分离行业的重要性。配体的发现依赖于合理设计技术的实施，这为新型亲和配体的工程设计提供了基础。亲和配体设计的主要目标是发现或提高功能，如增加稳定性或选择性。然而，方法必须适应当前的需求，即正在开发的生物制剂的数量和多样性，以及用于大数据分析和人工智能的新工具的可用性。在这篇综述中，我们概述了用于生物分离的亲和配体的发展，包括从早期发现到目前和未来该领域的发展趋势的合理设计技术的演变。
