Raman Spectroscopy Lab, Department of Engineering and Architecture, University of Trieste, P.le Europa 1, 34100 Trieste, Italy.
Université de Reims Champagne-Ardenne, BioSpecT-EA7506, UFR de Pharmacie, 51 rue Cognacq-Jay, 51097 Reims, France.
Anal Chem. 2020 Mar 3;92(5):4053-4064. doi: 10.1021/acs.analchem.9b05658. Epub 2020 Feb 21.
Surface-enhanced Raman scattering (SERS) is a powerful and sensitive technique for the detection of fingerprint signals of molecules and for the investigation of a series of surface chemical reactions. Many studies introduced quantitative applications of SERS in various fields, and several SERS methods have been implemented for each specific application, ranging in performance characteristics, analytes used, instruments, and analytical matrices. In general, very few methods have been validated according to international guidelines. As a consequence, the application of SERS in highly regulated environments is still considered risky, and the perception of a poorly reproducible and insufficiently robust analytical technique has persistently retarded its routine implementation. Collaborative trials are a type of interlaboratory study (ILS) frequently performed to ascertain the quality of a single analytical method. The idea of an ILS of quantification with SERS arose within the framework of Working Group 1 (WG1) of the EU COST Action BM1401 Raman4Clinics in an effort to overcome the problematic perception of quantitative SERS methods. Here, we report the first interlaboratory SERS study ever conducted, involving 15 laboratories and 44 researchers. In this study, we tried to define a methodology to assess the reproducibility and trueness of a quantitative SERS method and to compare different methods. In our opinion, this is a first important step toward a "standardization" process of SERS protocols, not proposed by a single laboratory but by a larger community.
表面增强拉曼散射（SERS）是一种强大而灵敏的技术，可用于检测分子的指纹信号，并研究一系列表面化学反应。许多研究引入了 SERS 在各个领域的定量应用，并且已经为每个特定应用实施了几种 SERS 方法，其性能特征、分析物使用、仪器和分析基质各不相同。一般来说，很少有方法根据国际准则进行验证。因此，SERS 在高度监管环境中的应用仍被认为存在风险，而且人们对可重复性差和不够稳健的分析技术的看法一直阻碍了其常规应用。协作试验是一种常用的实验室间研究（ILS），用于确定单一分析方法的质量。带有 SERS 的定量 ILS 的想法是在欧盟 COST 行动 BM1401 Raman4Clinics 的工作组 1（WG1）的框架内提出的，旨在克服对定量 SERS 方法的有问题的看法。在这里，我们报告了首次进行的实验室间 SERS 研究，涉及 15 个实验室和 44 名研究人员。在这项研究中，我们试图定义一种评估定量 SERS 方法重现性和准确性的方法，并比较不同的方法。在我们看来，这是迈向 SERS 协议“标准化”过程的重要第一步，不是由单个实验室提出，而是由更大的社区提出。
