Grabska-Zielińska Sylwia, Sionkowska Alina, Reczyńska Katarzyna, Pamuła Elżbieta
Department of Biomaterials and Cosmetic Chemistry, Faculty of Chemistry, Nicolaus Copernicus University in Toruń, 87-100 Toruń, Poland.
Department of Physical Chemistry and Polymer Physical Chemistry, Faculty of Chemistry, Nicolaus Copernicus University in Toruń, 87-100 Toruń, Poland.
Polymers (Basel). 2020 Feb 7;12(2):372. doi: 10.3390/polym12020372.
In this study, three-dimensional (3D) biopolymeric scaffolds made from collagen, silk fibroin and chitosan were successfully prepared by the freeze drying method. Dialdehyde starch (DAS) was used as a cross-linking agent for the materials. The properties of the materials were studied using density and porosity measurements, scanning electron microscope (SEM) imaging, swelling and moisture content measurements. Additionally, cytocompatibility of the materials in contact with MG-63 osteoblast-like cells was tested by live/dead staining and resazurin reduction assay on days 1, 3 and 7. It was found that new 3D materials made from collagen/silk fibroin/chitosan binary or ternary mixtures are hydrophilic with a high swelling ability (swelling rate in the range of 1680-1900%). Cross-linking of such biopolymeric materials with DAS increased swelling rate up to about 2100%, reduced porosity from 96-97% to 91-93%, and also decreased density and moisture content of the materials. Interestingly, presence of DAS did not influence the microstructure of the scaffolds as compared to non-cross-linked samples as shown by SEM. All the tested samples were found to be cytocompatible and supported adhesion and growth of MG-63 cells as shown by live-dead staining and metabolic activity test.
在本研究中，通过冷冻干燥法成功制备了由胶原蛋白、丝素蛋白和壳聚糖制成的三维（3D）生物聚合物支架。二醛淀粉（DAS）用作材料的交联剂。使用密度和孔隙率测量、扫描电子显微镜（SEM）成像、溶胀和水分含量测量来研究材料的性能。此外，在第1、3和7天通过活/死染色和刃天青还原试验测试了材料与MG-63成骨样细胞接触时的细胞相容性。结果发现，由胶原蛋白/丝素蛋白/壳聚糖二元或三元混合物制成的新型3D材料具有亲水性，溶胀能力高（溶胀率在1680-1900%范围内）。这种生物聚合物材料与DAS交联后，溶胀率提高到约2100%，孔隙率从96-97%降低到91-93%，同时材料的密度和水分含量也降低。有趣的是，如SEM所示，与未交联的样品相比，DAS的存在并未影响支架的微观结构。通过活/死染色和代谢活性测试表明，所有测试样品均具有细胞相容性，并支持MG-63细胞的粘附和生长。
