Department of Chemistry of Biomaterials and Cosmetics, Faculty of Chemistry, Nicolaus Copernicus University in Toruń, Poland.
Biomed Mater. 2017 Dec 15;13(1):015016. doi: 10.1088/1748-605X/aa8910.
In this study the influence of the addition of dialdehyde starch on the properties of scaffolds based on gelatin and chitosan obtained by the freeze-drying method was investigated. In addition, the adhesion and proliferation of human osteosarcoma SaOS-2 cells on the obtained scaffolds was examined. Chitosan and gelatin were mixed in different weight ratios (75/25, 50/50, 25/75) with 1, 2 and 5 wt% addition of dialdehyde starch. The obtained scaffolds were subjected to mechanical testing, infrared spectroscopy, swelling measurements, low-pressure porosimetry and zeta potential measurement. Internal material structures were observed by scanning electron microscopy. The results showed that the cross-linking process occurred after the addition of dialdehyde starch and resulted in increased mechanical strength, swelling properties, zeta potential and porosity of studied materials. The attachment of SaOS-2 cells to all modified materials was better compared to an unmodified control and the proliferation of these cells was markedly increased on modified scaffolds.
本研究考察了通过冷冻干燥法制备的明胶和壳聚糖支架中添加双醛淀粉对其性能的影响。此外，还研究了人骨肉瘤 SaOS-2 细胞在获得的支架上的黏附和增殖情况。壳聚糖和明胶以不同的质量比（75/25、50/50、25/75）与 1、2 和 5wt%的双醛淀粉混合。对所得支架进行力学性能测试、红外光谱、溶胀测量、低压孔隙率和zeta 电位测量。通过扫描电子显微镜观察内部材料结构。结果表明，添加双醛淀粉后发生了交联过程，导致研究材料的机械强度、溶胀性能、zeta 电位和孔隙率增加。与未改性对照相比，SaOS-2 细胞在所有改性材料上的黏附情况更好，这些细胞在改性支架上的增殖明显增加。
