Department of Chemistry of Biomaterials and Cosmetics, Faculty of Chemistry, Nicolaus Copernicus University in Torun, Poland.
Department of Chemistry of Biomaterials and Cosmetics, Faculty of Chemistry, Nicolaus Copernicus University in Torun, Poland.
Int J Biol Macromol. 2018 Feb;107(Pt A):470-477. doi: 10.1016/j.ijbiomac.2017.09.017. Epub 2017 Sep 7.
Scaffolds based on chitosan, collagen and hyaluronic acid, cross-linked by dialdehyde starch were obtained through the freeze-drying method. The porous structures were used as matrixes for calcium phosphate in situ precipitation. Composites were characterized by different analyses, e.g. infrared spectroscopy, SEM images, porosity, density, and mechanical tests. Moreover, an examination involving the energy dispersive X-ray spectroscopic method was carried out for the calcium and phosphorus ratio determination. In addition, the adhesion and proliferation of human osteosarcoma SaOS-2 cells were examined on the obtained scaffolds. The results showed that the properties of the scaffolds based on chitosan, collagen, and hyaluronic acid can be modified by dialdehyde starch addition. The mechanical parameters (i.e. compressive modulus and maximum compressive force), porosity, and density of the material were improved. Calcium phosphate was deposited in the scaffolds at the Ca/P ratio ∼2. SEM images showed the homogeneous structure, with interconnected pores. The cross-linker addition and an inorganic compound precipitation improved the biocompatibility of the scaffolds. The obtained materials can provide the support required in tissue engineering and regenerative medicine.
采用冷冻干燥法制备了壳聚糖、胶原和透明质酸为基础，双醛淀粉交联的支架。多孔结构用作磷酸钙原位沉淀的基质。通过不同的分析方法，如红外光谱、SEM 图像、孔隙率、密度和力学测试对复合材料进行了表征。此外，还进行了能量色散 X 射线能谱法分析以确定钙磷比。此外，还在获得的支架上检测了人骨肉瘤 SaOS-2 细胞的黏附和增殖。结果表明，通过添加双醛淀粉可以修饰基于壳聚糖、胶原和透明质酸的支架的性能。材料的力学参数（即压缩模量和最大压缩力）、孔隙率和密度得到了提高。在支架中以 Ca/P 比约 2 的比例沉积了磷酸钙。SEM 图像显示了均匀的结构，具有相互连通的孔。交联剂的添加和无机化合物的沉淀提高了支架的生物相容性。所得材料可为组织工程和再生医学提供所需的支持。
