State Key Laboratory of Reproductive Regulation & Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot 010070, China.
College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
Int J Mol Sci. 2020 Feb 7;21(3):1121. doi: 10.3390/ijms21031121.
Transgenic technology has huge application potential in agriculture and medical fields, such as producing new livestock varieties with new valuable features and xenotransplantation. However, how an exogenous gene affects the host animal's gene regulation networks and their health status is still poorly understood. In the current study, transgenic sheep were generated, and the tissues from 100-day abnormal (DAF_1) and normal (DAF_2) fetuses, postnatal lambs (DAF_4), transgenic-silencing (DAFG5), and -expressing (DAFG6) skin cells were collected and subjected to transcriptome sequencing, and their gene expression profiles were compared in multiple dimensions. The results were as follows. For DAF_1, its abnormal development was caused by pathogen invasion but not the introduction of the gene. expression down-regulated the genes related to the cell cycle; the NF-κB signaling pathway and the PI3K/Akt signaling pathway were down-regulated, and the PUFAs (polyunsaturated fatty acids) biosynthesis pathway was shifted toward the biosynthesis of high-level -3 LC-PUFAs (long-chain PUFAs). Four key node genes, , , , and were found to be responsible for the gene expression profile shift from the transgenic 100-day fetus to postnatal lamb, and may play a key role in the accumulation of -3 LC-PUFAs in transgenic sheep muscle. Our study provides new insights into the FUFAs synthesis regulation in transgenic animals.
转基因技术在农业和医学领域具有巨大的应用潜力，例如生产具有新有价值特征的新型家畜品种和异种移植。然而，外源基因如何影响宿主动物的基因调控网络及其健康状况仍知之甚少。在本研究中，我们生成了转基因绵羊，并收集了 100 日龄异常（DAF_1）和正常（DAF_2）胎儿、产后羔羊（DAF_4）、转基因沉默（DAFG5）和表达（DAFG6）皮肤细胞的组织，进行转录组测序，并在多个维度上比较了它们的基因表达谱。结果如下。对于 DAF_1，其异常发育是由病原体入侵引起的，而不是引入 基因所致。 表达下调了与细胞周期相关的基因；NF-κB 信号通路和 PI3K/Akt 信号通路下调，多不饱和脂肪酸（PUFAs）生物合成途径向高水平 -3 LC-PUFAs（长链 PUFAs）的生物合成方向转变。发现四个关键节点基因 、 、 、 和 负责从转基因 100 日龄胎儿到产后羔羊的基因表达谱转变， 可能在转基因绵羊肌肉中 -3 LC-PUFAs 的积累中发挥关键作用。我们的研究为转基因动物中 FUFAs 的合成调控提供了新的见解。
