Department of Natural Sciences, School of Science and the Environment, Manchester Metropolitan University, Manchester, UK.
Sci Rep. 2020 Feb 11;10(1):2316. doi: 10.1038/s41598-020-59048-6.
Interactions between bacteria govern the progression of respiratory infections; however, the mechanisms underpinning these interactions are still unclear. Understanding how a bacterial species comes to dominate infectious communities associated with respiratory infections has direct relevance to treatment. In this study, Burkholderia, Pseudomonas, and Staphylococcus species were isolated from the sputum of an individual with Cystic Fibrosis and assembled in a fully factorial design to create simple microcosms. Measurements of growth and habitat modification were recorded over time, the later using proton Nuclear Magnetic Resonance spectra. The results showed interactions between the bacteria became increasingly neutral over time. Concurrently, the bacteria significantly altered their ability to modify the environment, with Pseudomonas able to utilise secondary metabolites produced by the other two isolates, whereas the reverse was not observed. This study indicates the importance of including data about the habitat modification of a community, to better elucidate the mechanisms of bacterial interactions.
细菌之间的相互作用控制着呼吸道感染的进展；然而，这些相互作用的机制仍不清楚。了解一种细菌如何主导与呼吸道感染相关的感染性群落与治疗直接相关。在这项研究中，从患有囊性纤维化的个体的痰中分离出伯克霍尔德菌、假单胞菌和葡萄球菌，并以完全析因设计组装，以创建简单的微宇宙。随着时间的推移记录了生长和栖息地修饰的测量值，后者使用质子核磁共振光谱。结果表明，随着时间的推移，细菌之间的相互作用变得越来越中性。同时，细菌显著改变了它们修饰环境的能力，假单胞菌能够利用其他两种分离株产生的次生代谢物，而反之则没有观察到。这项研究表明，将有关群落栖息地修饰的信息纳入其中对于更好地阐明细菌相互作用的机制非常重要。
