School of Chemistry and Chemical Engineering, Yancheng Institute of Technology, Yancheng 224051, People's Republic of China.
Department of Chemistry, University of California, Riverside, California 92521, United States.
Anal Chem. 2020 Mar 3;92(5):4094-4100. doi: 10.1021/acs.analchem.9b05762. Epub 2020 Feb 21.
Selectivity is a crucial parameter for photoelectrochemical (PEC) sensing in a practical setting. Despite the use of specific probes such as aptamers, antibodies, and enzymes, coexisting interferences can still result in inaccuracies in PEC sensing, especially for complex biosample matrixes. Here we report the design of an FeO@SiO@TiO magnetic-optical bifunctional beacon applied in a novel PEC sensor that can selectively capture progesterone in complex biosamples, be magnetically separated and cleaned, and be detected in pure phosphate buffer solution (PBS). The magnetic separation strategy efficiently removes the complex coexisting species from the modified electrode surface and drastically enhances the selectivity of the as-designed PEC sensor. The as-designed PEC sensor is cost-effective, easy to fabricate, highly selective and sensitive, and highly reliable, making it a promising platform for efficient aptasensing.
选择性是实际应用中光电化学（PEC）传感的一个关键参数。尽管使用了特定的探针，如适体、抗体和酶，但共存的干扰仍然会导致 PEC 传感的不准确，特别是对于复杂的生物样本基质。在这里，我们报告了一种 FeO@SiO@TiO 磁性-光学双功能信标的设计，该信标应用于一种新型的 PEC 传感器中，可以在复杂的生物样本中选择性地捕获孕酮，通过磁性分离和清洗，并在纯磷酸盐缓冲溶液（PBS）中进行检测。磁性分离策略有效地从修饰电极表面去除了复杂的共存物质，极大地提高了所设计的 PEC 传感器的选择性。所设计的 PEC 传感器具有成本效益、易于制造、高选择性和灵敏度以及高度可靠性，使其成为一种有前途的高效适体传感平台。
