Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba-ku, Sendai, 980-8578, Japan.
Angew Chem Int Ed Engl. 2020 May 11;59(20):7755-7760. doi: 10.1002/anie.201915846. Epub 2020 Mar 6.
We propose a label-free method for measuring intracellular temperature using a Raman image of a cell in the O-H stretching band. Raman spectra of cultured cells and the medium were first measured at various temperatures using a Raman microscope and the intensity ratio of the two regions of the O-H stretching band was calculated. The intensity ratio varies linearly with temperature in both the medium and cells, and the resulting calibration lines allow simultaneous visualization of both intracellular and extracellular temperatures in a label-free manner. We applied this method to the measurement of temperature changes after the introduction of FCCP (carbonyl cyanide-p-trifluoromethoxyphenylhydrazone) in living cells. We observed a temperature rise in the cytoplasm and succeeded in obtaining an image of the change in intracellular temperature after the FCCP treatment.
我们提出了一种使用细胞在 O-H 伸缩带的拉曼图像来测量细胞内温度的无标记方法。首先使用拉曼显微镜在不同温度下测量培养细胞和培养基的拉曼光谱，并计算 O-H 伸缩带两个区域的强度比。在培养基和细胞中，强度比随温度呈线性变化，校准线可以同时以非标记的方式可视化细胞内和细胞外的温度。我们将该方法应用于活细胞中引入 FCCP（羰基氰化物-对三氟甲氧基苯腙）后温度变化的测量。我们观察到细胞质温度升高，并成功获得了 FCCP 处理后细胞内温度变化的图像。
