Universidad Nacional de San Martin-Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Investigación e Ingeniería Ambiental, Ecología de Enfermedades Transmitidas por Vectores (2eTV), San Martín, Provincia de Buenos Aires, Argentina.
Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina.
Mem Inst Oswaldo Cruz. 2020 Feb 7;115:e190390. doi: 10.1590/0074-02760190390. eCollection 2020.
The mosquito Culex pipiens s.s. L. occurs as two bioforms that differ in physiology and behaviour affecting virus transmission cycles. To assess the occurrence of Cx. pipiens bioforms in the southernmost limit of its distribution, specimens were collected aboveground in southern Buenos Aires Province and east Patagonia, Argentina. Ten larvae and 25 adults were individually processed and identified by polymerase chain reaction (PCR) amplification of Ace-2 and CQ11 loci. Culex quinquefasciatus Say (one larva, two adults), Cx. pipiens f. molestus (one larva, one adult) and one adult of hybrid origin were identified in Buenos Aires Province; only Cx. pipiens f. molestus was recorded in Patagonia (eight larvae, 21 adults). The potential absence of bioform pipiens and its implications in arbovirus enzootic cycles is discussed.
致倦库蚊（Culex pipiens s.s. L.）存在两种生物型，它们在生理和行为上存在差异，从而影响病毒的传播周期。为了评估致倦库蚊生物型在其分布最南端的出现情况，在阿根廷南部布宜诺斯艾利斯省和东巴塔哥尼亚采集了地面以上的标本。用聚合酶链反应（PCR）扩增 Ace-2 和 CQ11 基因座，对 10 只幼虫和 25 只成蚊进行了单独处理和鉴定。在布宜诺斯艾利斯省鉴定出了库蚊（1 只幼虫，2 只成蚊）、致倦库蚊亚种（1 只幼虫，1 只成蚊）和 1 只杂交来源的成蚊；在巴塔哥尼亚仅记录到致倦库蚊亚种（8 只幼虫，21 只成蚊）。讨论了生物型 pipiens 的潜在缺失及其对虫媒病毒地方性循环的影响。
