Instituto de Investigaciones en Biodiversidad y Biotecnología (INBIOTEC - CONICET); Fundación Para Investigaciones Biológicas Aplicadas (FIBA), Vieytes 3103, Mar del Plata, Argentina.
Departamento de Biología - Instituto y Museo de Ciencias Naturales, Facultad de Ciencias Exactas, Físicas y Naturales, Universidad Nacional de San Juan, CONICET, San Juan, Argentina.
Sci Rep. 2021 Jan 13;11(1):1094. doi: 10.1038/s41598-020-80034-5.
In an attempt to evaluate the susceptibility of the mosquito Culex quinquefasciatus to bacterial agents, a population naturally infected with a Wolbachia pipientis wPipSJ native strain was tested against the action of three bacterial mosquitocides, Bacillus thuringiensis subsp. israelensis, Bacillus wiedmannii biovar thuringiensis and Lysinibacillus sphaericus. Tests were carried out on mosquito larvae with and without Wolbachia (controls). Cx. quinquefasciatus naturally infected with the native wPipSJ strain proved to be more resistant to the pathogenic action of the three mosquitocidal bacterial strains. Additionally, wPipSJ was fully characterised using metagenome-assembled genomics, PCR-RFLP (PCR-Restriction Fragment Length Polymorphism) and MLST (MultiLocus Sequence Typing) analyses. This Wolbachia strain wPipSJ belongs to haplotype I, group wPip-III and supergroup B, clustering with other mosquito wPip strains, such as wPip PEL, wPip JHB, wPip Mol, and wAlbB; showing the southernmost distribution in America. The cytoplasmic incompatibility phenotype of this strain was revealed via crosses between wildtype (Wolbachia) and antibiotic treated mosquito populations. The results of the tests with the bacterial agents suggest that Cx. quinquefasciatus naturally infected with wPipSJ is less susceptible to the pathogenic action of mosquitocidal bacterial strains when compared with the antibiotic-treated mosquito isoline, and is more susceptible to B. thuringiensis subsp. israelensis than to the other two mosquitocidal agents.
为了评估蚊子库蚊对细菌制剂的敏感性，对一种自然感染沃尔巴克氏体 pipientis wPipSJ 本土株的种群进行了测试，以评估三种细菌杀蚊剂的作用，苏云金芽孢杆菌亚种。以色列、魏德曼氏芽孢杆菌生物变种苏云金芽孢杆菌和球形芽孢杆菌。在有和没有沃尔巴克氏体（对照）的蚊子幼虫上进行了测试。自然感染本土 wPipSJ 株的库蚊幼虫对三种杀蚊细菌菌株的致病作用表现出更强的抗性。此外，还利用宏基因组组装基因组学、PCR-RFLP（PCR-限制性片段长度多态性）和 MLST（多位点序列分型）分析对 wPipSJ 进行了全面表征。这种沃尔巴克氏体菌株 wPipSJ 属于 I 单倍型、wPip-III 组和 B 超级群，与其他蚊子 wPip 菌株（如 wPip PEL、wPip JHB、wPip Mol 和 wAlbB）聚类；在美洲表现出最南端的分布。通过在野生型（沃尔巴克氏体）和抗生素处理的蚊子种群之间进行杂交，揭示了该菌株的细胞质不兼容表型。与抗生素处理的蚊子分离株相比，感染 wPipSJ 的蚊子对细菌制剂的敏感性降低，并且对苏云金芽孢杆菌亚种。以色列比其他两种杀蚊剂更敏感。
