Sato Ana Paula, Vaz Frederico Fontanelli, Konell Aline Luiza, Koch Marilia de Oliveira, Ferreira Rafaela Furioso, Sipinski Elenise Angelotti Bastos, Dittrich Rosangela Locatelli
Programa de Pós-graduação em Ciências Veterinárias, Universidade Federal do Paraná - UFPR, Curitiba, PR, Brasil.
Programa de Pós-graduação em Patologia Experimental e Comparada, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo - USP, São Paulo, SP, Brasil.
Rev Bras Parasitol Vet. 2020 Feb 10;29(1):e017519. doi: 10.1590/S1984-29612019107. eCollection 2020.
Toxoplasma gondii, Neospora caninum and Sarcocystis neurona are obligate intracellular parasites within the phylum Apicomplexa. The red-tailed Amazon parrot (Amazona brasiliensis) is a near-threatened species of psittacine that is endemic to the Atlantic Forest of Brazil and has been designated as a bioindicator because of its sensitivity to environmental qualitative status and changes. The aim of this study was to evaluate the presence of antibodies against T. gondii, N. caninum and S. neurona in wild red-tailed Amazon parrot nestlings on Rasa Island, Brazil. Blood samples were collected from 51 parrots and plasma samples were stored at - 20 °C until immunofluorescence antibody tests (IFAT) were performed. Antigen slides were prepared using tachyzoites of T. gondii (RH strain) and, N. caninum (NC-1 strain) and using merozoites of S. neurona (SNR37 strain). Plasma samples were tested at initial dilutions of 1:16 for T. gondii, 1:50 for N. caninum and 1:5 for S. neurona. An anti-chicken antibody conjugated with FITC was used as a secondary antibody at 1:50 dilution. No antibodies for any of these three protozoa were found, thus suggesting that these wild red-tailed Amazon parrot nestlings had not been exposed to these parasites.
刚地弓形虫、犬新孢子虫和神经肉孢子虫是顶复门中的专性细胞内寄生虫。红尾亚马逊鹦鹉（Amazona brasiliensis）是一种近危鹦鹉物种，原产于巴西大西洋森林，因其对环境质量状况和变化敏感而被指定为生物指示物种。本研究的目的是评估巴西拉萨岛上野生红尾亚马逊鹦鹉雏鸟体内抗刚地弓形虫、犬新孢子虫和神经肉孢子虫抗体的存在情况。从51只鹦鹉身上采集血液样本，血浆样本储存在-20°C直至进行免疫荧光抗体试验（IFAT）。使用刚地弓形虫（RH株）速殖子、犬新孢子虫（NC-1株）和神经肉孢子虫（SNR37株）裂殖子制备抗原玻片。血浆样本针对刚地弓形虫的初始稀释度为1:16、针对犬新孢子虫为1:50、针对神经肉孢子虫为1:5进行检测。与异硫氰酸荧光素（FITC）偶联的抗鸡抗体以1:50稀释度用作二抗。未发现针对这三种原生动物中任何一种的抗体，因此表明这些野生红尾亚马逊鹦鹉雏鸟未接触过这些寄生虫。
