Department of Food Science, University of Guelph, Guelph, ON, N1G2W1, Canada.
Institute for Health Care Science, Suntory Wellness Limited, 8-1-1 Seikadai, Seika-cho, Soraku-gun, Kyoto, 619-0284, Japan.
Mol Immunol. 2020 Apr;120:43-51. doi: 10.1016/j.molimm.2020.01.025. Epub 2020 Feb 9.
Food protein-induced allergies are primarily aggravated due to imbalance immune responses. Earlier studies by different research groups have reported that the intervention of Lactobacillus pentosus (L. pentosus) S-PT84 can modulate T-helper (Th)1/Th2 balance through regulatory T cells and can effectively promote type 1 immunity by activating dendritic cells and natural killer cells, such biological activity makes L. pentosus S-PT84 a potential mediation in controlling food allergy. Thus, this study aimed to evaluate the effects of L. pentosus S-PT84 against egg ovalbumin (OVA)-induced allergic response in mice. BALB/c mice (n = 12/group) were sensitized with OVA (50 μg/mice) via intraperitoneal injection (IP) for four weeks and subsequently administered with three different doses of L. pentosus S-PT84 via pelleted diet. The allergenic status was assessed by clinical signs, serum histamine, mouse mast cell protease (MMCP) level, and antibody activity, cytokines level in splenocytes, and expression of T regulatory cells (T-regs) in blood. The intervention of L. pentosus S-PT84, precisely at the high dose (0.6 % L. pentosus S-PT84 in pelleted diet) group, significantly reduced the clinical allergenic symptoms and reduced the histamine and MMCP levels in serum. However, the intervention of L. pentosus S-PT84 did not affect the OVA-specific IgE, IgG concentration, but led to lower the total IgE and IgG titers, suggesting that the therapeutic effect of L. pentosus S-PT84 may be due to development of immune tolerance. Moreover, differences in the immune response were observed after L. pentosus S-PT84 intervention, as it significantly reduced the production of IL-4, IL-17, and increased the population of CD25Foxp3 cells. Thus, it can be concluded that the intervention of L. pentosus S-PT84 provides a potential therapeutic strategy to reduce the chicken egg OVA-induced allergic symptoms.
食物蛋白诱导的过敏主要是由于免疫反应失衡加剧的。不同研究小组的早期研究报告称，植物乳杆菌（L. pentosus）S-PT84 的干预可以通过调节性 T 细胞调节辅助性 T 细胞（Th）1/Th2 平衡，并通过激活树突状细胞和自然杀伤细胞有效促进 1 型免疫，这种生物活性使 L. pentosus S-PT84 成为控制食物过敏的潜在调节剂。因此，本研究旨在评估植物乳杆菌 S-PT84 对卵清蛋白（OVA）诱导的小鼠过敏反应的影响。BALB/c 小鼠（每组 12 只）通过腹腔注射（IP）用 OVA（50μg/只）致敏四周，随后通过颗粒饲料给予三种不同剂量的植物乳杆菌 S-PT84。通过临床症状、血清组胺、鼠肥大细胞蛋白酶（MMCP）水平和抗体活性、脾细胞细胞因子水平以及血液中调节性 T 细胞（T-regs）的表达来评估变应原状态。植物乳杆菌 S-PT84 的干预，准确地在高剂量（颗粒饲料中 0.6%的植物乳杆菌 S-PT84）组，显著减少了临床变应性症状，并降低了血清中的组胺和 MMCP 水平。然而，植物乳杆菌 S-PT84 的干预并不影响 OVA 特异性 IgE、IgG 浓度，但导致总 IgE 和 IgG 滴度降低，表明植物乳杆菌 S-PT84 的治疗效果可能是由于免疫耐受的发展。此外，在植物乳杆菌 S-PT84 干预后观察到免疫反应的差异，因为它显著降低了 IL-4、IL-17 的产生，并增加了 CD25Foxp3 细胞的数量。因此，可以得出结论，植物乳杆菌 S-PT84 的干预为减少鸡卵 OVA 诱导的过敏症状提供了一种潜在的治疗策略。
