一种保守的羧酸酯酶抑制（TMV）在植物中的积累。
A Conserved Carboxylesterase Inhibits (TMV) Accumulation in Plants.
机构信息
Department of Biological Sciences, National University of Singapore, Singapore 117543, Singapore.
National University of Singapore (Suzhou) Research Institute, Suzhou 215123, China.
出版信息
Viruses. 2020 Feb 10;12(2):195. doi: 10.3390/v12020195.
A carboxylesterase (CXE) or carboxylic-ester hydrolase is an enzyme that catalyzes carboxylic ester and water into alcohol and carboxylate. In plants, CXEs have been implicated in defense, development, and secondary metabolism. We discovered a new CXE gene in that is related to virus resistance. The transcriptional level of NbCXE expression was significantly increased after (TMV) infection. Transient over-expression of NbCXE inhibited TMV accumulation in plants. Conversely, when the NbCXE gene was silenced with a (TRV)-based gene silencing system, TMV RNA accumulation was increased in NbCXE-silenced plants after infection. NbCXE protein was shown to interact with TMV coat protein (CP in vitro. Additionally, the expressions of host defense-related genes were increased in transient NbCXE-overexpressed plants but decreased in NbCXE silenced plants. In summary, our study showed that NbCXE is a novel resistance-related gene involved in host defense responses against TMV infection.
一种羧酸酯酶（CXE）或羧酸酯水解酶是一种能催化羧酸酯和水生成醇和羧酸盐的酶。在植物中，CXE 被认为与防御、发育和次生代谢有关。我们在 中发现了一个与抗病毒有关的新 CXE 基因。在 （TMV）感染后，NbCXE 的转录水平显著增加。瞬时过表达 NbCXE 抑制了 植物中 TMV 的积累。相反，当使用基于 （TRV）的基因沉默系统沉默 NbCXE 基因时，NbCXE 沉默的植物在感染后 TMV RNA 的积累增加。NbCXE 蛋白被证明在体外与 TMV 外壳蛋白（CP 相互作用。此外，在瞬时过表达 NbCXE 的植物中，与宿主防御相关的基因表达增加，而在 NbCXE 沉默的 植物中则减少。总之，我们的研究表明，NbCXE 是一个新的与抗病毒有关的基因，参与了对 TMV 感染的宿主防御反应。
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