Yan Yaxing, Jiang Mei, Wang Xiao
Shandong Engineering Research Center for Innovation and Application of General Technology for Separation of Natural Products, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250014, China.
Key Laboratory for Natural Active Pharmaceutical Constituents Research in Universities of Shandong Province, School of Pharmaceutical Sciences, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250014, China.
Plant Cell Rep. 2025 Jan 30;44(2):45. doi: 10.1007/s00299-025-03426-9.
GeCXE9 can catalyze the hydrolysis of parishin A via two pathways during the medicinal processing of Gastrodia elata. Gastrodia elata Bl. is used in traditional Chinese medicine for its bioactive compounds, particularly phenols. The molecular mechanisms of phenols formation and regulation remain to be fully explored. Here, we identified 13 GeCXE genes in G. elata. These members were mapped to eight chromosomes and possessed motifs necessary for catalysis. Phylogenetic analyses grouped them into four categories, supported by gene structure and motif composition. The cis-acting elements analysis indicated most GeCXEs contained elements associated with plant hormones or stress response. In vitro expression experiments revealed that GeCXE9 can catalyze the hydrolysis of parishin A via two pathways, producing parishin B and parishin C, respectively. Whereas in the absence of GeCXE9, parishin A is hydrolyzed solely to parishin B. In conclusion, GeCXE9 plays a crucial role in determining the metabolic pathways of phenols in G. elata, and its discovery provides a viable approach for the artificial regulation of phenols in this species.
在天麻的炮制过程中，GeCXE9可通过两条途径催化 parishin A 的水解。天麻因其生物活性化合物，特别是酚类物质，而被用于传统中药。酚类物质形成和调控的分子机制仍有待充分探索。在此，我们在天麻中鉴定出13个 GeCXE 基因。这些成员被定位到8条染色体上，并拥有催化所需的基序。系统发育分析将它们分为四类，基因结构和基序组成也支持这一分类。顺式作用元件分析表明，大多数 GeCXE 含有与植物激素或应激反应相关的元件。体外表达实验表明，GeCXE9 可通过两条途径催化 parishin A 的水解，分别产生 parishin B 和 parishin C。而在没有 GeCXE9 的情况下，parishin A 仅水解为 parishin B。总之，GeCXE9 在决定天麻中酚类物质的代谢途径中起关键作用，其发现为该物种中酚类物质的人工调控提供了一种可行的方法。
