Novikova Galina V, Stepanchenko Natalia S, Zorina Anna A, Nosov Alexander V, Rakitin Victor Y, Moshkov Igor E, Los Dmitry A
K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya street 35, Moscow 127276, Russia.
Life (Basel). 2020 Feb 10;10(2):15. doi: 10.3390/life10020015.
Recent studies indicate direct links between molecular cell cycle and cell differentiation machineries. Ethylene and abscisic acid (ABA) are known to affect cell division and differentiation, but the mechanisms of such effects are poorly understood. As ethylene and ABA signaling routes may interact, we examined their involvement in cell division and differentiation in cell tissue cultures derived from several plants: wild type (Col-0), and ethylene-insensitive mutants and . We designed an experimental setup to analyze the growth-related parameters and molecular mechanisms in proliferating cells upon short exposure to ABA. Here, we provide evidence for the ethylene-ABA signaling pathways' interaction in the regulation of cell division and differentiation as follows: (1) when the ethylene signal transduction pathway is functionally active (Col-0), the cells actively proliferate, and exogenous ABA performs its function as an inhibitor of DNA synthesis and division; (2) if the ethylene signal is not perceived (etr1-1), then, in addition to cell differentiation (tracheary elements formation), cell death can occur. The addition of exogenous ABA can rescue the cells via increasing proliferation; (3) if the ethylene signal is perceived, but not transduced (ein2-1), then cell differentiation takes place-the latter is enhanced by exogenous ABA while cell proliferation is reduced; (4) when the signal transduction pathway is constitutively active, the cells begin to exit the cell cycle and proceed to endo-reduplication (ctr1-1). In this case, the addition of exogenous ABA promotes reactivation of cell division.
近期研究表明分子细胞周期与细胞分化机制之间存在直接联系。已知乙烯和脱落酸（ABA）会影响细胞分裂和分化，但其作用机制尚不清楚。由于乙烯和ABA信号通路可能相互作用，我们研究了它们在几种植物来源的细胞组织培养物中的细胞分裂和分化过程中的作用：野生型（Col-0）、乙烯不敏感突变体和。我们设计了一个实验装置，以分析短期暴露于ABA后增殖细胞中与生长相关的参数和分子机制。在此，我们为乙烯-ABA信号通路在细胞分裂和分化调控中的相互作用提供了如下证据：（1）当乙烯信号转导通路功能活跃时（Col-0），细胞积极增殖，外源性ABA发挥其作为DNA合成和分裂抑制剂的功能；（2）如果乙烯信号未被感知（etr1-1），那么，除了细胞分化（管状分子形成）外，还可能发生细胞死亡。添加外源性ABA可通过增加增殖来挽救细胞；（3）如果乙烯信号被感知但未被转导（ein2-1），则会发生细胞分化——外源性ABA会增强细胞分化，同时细胞增殖减少；（4）当信号转导通路组成型激活时，细胞开始退出细胞周期并进行内复制（ctr1-1）。在这种情况下，添加外源性ABA可促进细胞分裂的重新激活。
