Department of General Surgery, The Second Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong, PR China.
Department of Surgical Oncology, Shantou Central Hospital, Shantou, Guangdong, PR China.
Epigenomics. 2020 Apr;12(7):587-603. doi: 10.2217/epi-2019-0404. Epub 2020 Feb 13.
The role of circRNAs in esophageal squamous cell cancer (ESCC) remains unclear. Here we profiled six pair plasma circRNA in ESCC based on RNA sequencing, and then verified the elevation of hsa_circ_0004771 in 20 cancer tissues and 105 pair case-control plasma samples by quantitative reverse transcriptase PCR. The upregulation of hsa_circ_0004771 was correlated with heavier tumor burden and poor prognosis, knockdown of it inhibited the ESCC cells proliferation both and . Mechanistically, hsa_circ_0004771 positively regulated CDC25A by acting as a molecular sponge of miR-339-5p and rescue assay confirmed this regulatory relationship. These results suggested that hsa_circ_0004771 can serve as a general less-invasive biomarker and may provide diagnostic and prognostic value in carcinoma.
环状 RNA 在食管鳞状细胞癌（ESCC）中的作用尚不清楚。 在这里，我们基于 RNA 测序对 ESCC 中的六种配对血浆环状 RNA 进行了分析，然后通过定量逆转录 PCR 在 20 个肿瘤组织和 105 对病例对照血浆样本中验证了 hsa_circ_0004771 的升高。 hsa_circ_0004771 的上调与肿瘤负荷加重和预后不良相关，其敲低抑制 ESCC 细胞的增殖。 机制上，hsa_circ_0004771 通过充当 miR-339-5p 的分子海绵正向调节 CDC25A，并通过挽救实验证实了这种调节关系。 这些结果表明 hsa_circ_0004771 可以作为一种一般的非侵入性生物标志物，并可能在癌症中提供诊断和预后价值。
