Laboratory of molecular epidemiology of viral infections, I.N. Blokhina Nizhny Novgorod Research Institute of Epidemiology and Microbiology, Nizhny Novgorod, Russian Federation.
Arch Virol. 2020 Apr;165(4):865-875. doi: 10.1007/s00705-020-04553-2. Epub 2020 Feb 12.
The G1P[8] genotype is one of the most common among rotaviruses circulating in the last 40 years. Therefore, this genotype is a component of rotavirus vaccines licensed throughout the world. This paper presents the results of a 35-year (1984-2019) observation of the circulation of G1P[8] rotaviruses among children under 14 in one region (Nizhny Novgorod, Russia) without vaccine pressure. Several complementary approaches were used: RNA electropherotyping by polyacrylamide gel electrophoresis, PCR genotyping, and cDNA sequencing of rotavirus VP4 and VP7 genes. A total of 8375 rotavirus-positive samples were examined, and the proportion of genotype G1P[8] rotaviruses was 39.9% (4.3-98.9%). Two cycles of high circulation activity (1984-1993 and 1993-2007) and one cycle of low activity (2007-2019) were noted. Phylogenetic analysis revealed the presence of rotaviruses of two VP4 gene lineages (P[8]-1 and P[8]-3) and two VP7 gene lineages (sublineages IA, IB, ID, II-B, II-C, and II- E). The prolonged circulation of rotaviruses of only one sublineage (G1-II-E) and then a change of the prevailing sublineage within the G1-II lineage (from E to C) during the active circulation were shown. Since 2011, when the circulation intensity of G1P[8] rotaviruses was low, the appearance of strains of the G1-I lineage and their co-circulation with strains of the G1-II lineage were observed in the population.
G1P[8] 基因型是过去 40 年中流行的轮状病毒之一。因此，该基因型是世界范围内许可使用的轮状病毒疫苗的组成部分。本文介绍了在没有疫苗压力的情况下，对一个地区（俄罗斯下诺夫哥罗德） 14 岁以下儿童中 G1P[8] 轮状病毒流行情况进行 35 年（1984-2019 年）观察的结果。使用了几种互补方法：聚丙烯酰胺凝胶电泳的 RNA 电泳图型、PCR 基因分型和轮状病毒 VP4 和 VP7 基因的 cDNA 测序。共检测了 8375 份轮状病毒阳性样本，G1P[8] 轮状病毒的比例为 39.9%（4.3-98.9%）。观察到两个高循环活动周期（1984-1993 年和 1993-2007 年）和一个低活动周期（2007-2019 年）。系统进化分析显示存在两种 VP4 基因谱系（P[8]-1 和 P[8]-3）和两种 VP7 基因谱系（亚谱系 IA、IB、ID、II-B、II-C 和 II-E）的轮状病毒。仅一种亚谱系（G1-II-E）的轮状病毒长期循环，然后在活跃循环期间 G1-II 谱系内流行的亚谱系发生变化（从 E 到 C）。自 2011 年 G1P[8] 轮状病毒循环强度较低以来，在人群中观察到 G1-I 谱系的菌株出现及其与 G1-II 谱系菌株的共同循环。
