Morozova Olga V, Sashina Tatiana A, Epifanova Natalia V, Kashnikov Alexander Yu, Novikova Nadezhda A
I.N. Blokhina Nizhny Novgorod Research Institute of Epidemiology and Microbiology, 71 Malaya Yamskaya Str., Nizhny Novgorod, Russian Federation, 603950.
Arch Virol. 2021 Jan;166(1):115-124. doi: 10.1007/s00705-020-04853-7. Epub 2020 Oct 20.
Rotavirus infection is one of the leading causes of acute gastroenteritis in children in their first years of life. We studied the genotypic diversity of rotavirus A (RVA) strains in Nizhny Novgorod, Russia, during the period 2016-19. In total, 4714 samples of faeces from children admitted to the Nizhny Novgorod Hospital for Infectious Diseases with acute gastroenteritis were examined. The share of rotavirus-positive samples was 31.5% in 2016-17. It decreased to 21.6% in 2018-19. In Nizhny Novgorod, all six global types of RVA were detected (G1P[8], G2P[4], G3P[8], G4P[8], G9P[8] and G12P[8]), as well as sporadic samples with genotypes G9P[4], G3P[9], G9P[9], G8P[8], G2P[8], G4P[4], G3P[9]. The fraction of strains with genotype G2P[4] gradually increased from 5.9% in 2016-17 to 39.1% in 2018-19. Simultaneously, the proportion of G9P[8] strains decreased from 63.2% to 27.7% in the same period. Phylogenetic analysis showed that rotaviruses with the G2P[4] genotype carried ubiquitous alleles of the VP7 and VP4 genes during the period of their prevalence: G2-IVa-1 and G2-IVa-3; P[4]-IVa and P[4]-IVb. As rotavirus vaccination is not widely used in the region because it is not included in the national vaccination calendar in Russia so far, the increase in the number of G2P[4] RVA is likely due to natural strain fluctuations.
轮状病毒感染是一岁以内儿童急性肠胃炎的主要病因之一。我们研究了2016年至2019年期间俄罗斯下诺夫哥罗德地区A组轮状病毒（RVA）毒株的基因多样性。总共检测了4714份因急性肠胃炎入住下诺夫哥罗德传染病医院的儿童粪便样本。2016 - 2017年，轮状病毒阳性样本的比例为31.5%。2018 - 2019年降至21.6%。在下诺夫哥罗德，检测到了所有六种全球流行的RVA类型（G1P[8]、G2P[4]、G3P[8]、G4P[8]、G9P[8]和G12P[8]），以及基因型为G9P[4]、G3P[9]、G9P[9]、G8P[8]、G2P[8]、G4P[4]、G3P[9]的散发病例样本。基因型为G2P[4]的毒株比例从2016 - 2017年的5.9%逐渐上升至2018 - 2019年的39.1%。同时，同期G9P[8]毒株的比例从63.2%降至27.7%。系统发育分析表明，基因型为G2P[4]的轮状病毒在流行期间携带普遍存在的VP7和VP4基因等位基因：G2-IVa-1和G2-IVa-3；P[4]-IVa和P[4]-IVb。由于轮状病毒疫苗在该地区未广泛使用，因为到目前为止它未被纳入俄罗斯国家疫苗接种计划，所以G2P[4] RVA数量的增加可能是由于自然毒株波动所致。
