Department of Microbiology, Chung-Ang University College of Medicine, Seoul, South Korea.
J Med Virol. 2010 May;82(5):886-96. doi: 10.1002/jmv.21653.
The human rotavirus G1P[8] strain is one of the most common rotaviruses worldwide, including Korea. Six Korean G1P[8] human rotaviruses, isolated using cell culture techniques, were characterized on the basis of sequence differences in VP7, VP4, VP6, and NSP4 genes to elucidate the evolutionary relationships in the community. All strains had a long RNA electropherotype, supported by VP6 gene analysis, clearly associated with subgroup II specificity. The phylogenetic analysis of VP7 gene sequences showed that they all clustered into lineage I, as reported for G1 strains in Japan, China, Vietnam, and Thailand. In addition, phylogenetic analysis of the VP4 gene showed that they belong to two distinct lineages, P[8]-II and P[8]-III. With respect to the NSP4 gene, all strains belonged to genotype B. An understanding of the ecology and molecular evolution of rotaviruses circulating in the country is very important for the development of vaccines and vaccination strategies. This study provides new information concerning the genetic variability of the rotavirus strain G1P[8] occurring most commonly as a vaccine candidate.
人轮状病毒 G1P[8] 株是全世界最常见的轮状病毒之一，包括韩国。本研究使用细胞培养技术从韩国分离到 6 株 G1P[8] 人轮状病毒，基于 VP7、VP4、VP6 和 NSP4 基因序列差异对其进行了特征分析，以阐明社区中的进化关系。所有毒株均具有长型 RNA 电泳图，VP6 基因分析支持其属于亚组 II 特异性。VP7 基因序列的系统进化分析表明，它们均聚类到日本、中国、越南和泰国报道的 G1 株谱系 I 中。此外，VP4 基因的系统进化分析表明，它们属于 P[8]-II 和 P[8]-III 两个不同的谱系。关于 NSP4 基因，所有毒株均属于基因型 B。了解在该国流行的轮状病毒的生态学和分子进化对于疫苗和接种策略的制定非常重要。本研究为最常见的疫苗候选株 G1P[8]轮状病毒的遗传变异性提供了新信息。
