在生产伊卡鲁霉素（5/6/5型）的底盘中对5/5/6型多环四酰胺大环内酯进行工程化生物合成。
Engineered Biosynthesis of 5/5/6 Type Polycyclic Tetramate Macrolactams in an Ikarugamycin (5/6/5 Type)-Producing Chassis.
作者信息
Jin Hongbo, Zhang Wenjun, Zhang Guangtao, Zhang Liping, Liu Wei, Zhang Changsheng
机构信息
Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, China.
University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing 100049, China.
出版信息
Org Lett. 2020 Mar 6;22(5):1731-1735. doi: 10.1021/acs.orglett.9b04672. Epub 2020 Feb 13.
Combinatorial biosynthesis of 5/5/6 type polycyclic tetramate macrolactams (PoTeMs) was achieved in an engineered ikarugamycin (5/6/5 type) producer by introducing a set of 5/5/6 type PoTeM biosynthetic genes from . This study supports the idea that PoTeMs share a common polyene tetramate precursor generated by the hybrid polyketide synthase/nonribosomal peptide synthetase enzymes. The X-ray crystal structure of pactamide G () sets an example for resolving the absolute configuration of 5/5/6 type PoTeMs.
通过从引入一组5/5/6型多环四酰胺大环内酯（PoTeMs）生物合成基因，在工程改造的伊卡鲁霉素（5/6/5型）产生菌中实现了5/5/6型多环四酰胺大环内酯的组合生物合成。这项研究支持了PoTeMs共享由混合聚酮合酶/非核糖体肽合成酶产生的常见多烯四酰胺前体的观点。帕卡酰胺G（）的X射线晶体结构为解析5/5/6型PoTeMs的绝对构型树立了一个典范。
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