Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR 97331.
Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC 27695.
Plant Dis. 2020 Apr;104(4):1167-1174. doi: 10.1094/PDIS-06-19-1285-RE. Epub 2020 Feb 13.
Grape powdery mildew (GPM) fungicide programs consist of 5 to 15 applications, depending on region or market, in an attempt to achieve the high fruit quality standards demanded by the market. Understanding how fungicides redistribute and targeting redistributing fungicide to critical crop phenological stages could improve fungicide protection of grape clusters. This study evaluated fungicide redistribution in grapevines from major fungicide groups labeled for GPM control. Translaminar and xylem redistribution was examined by placing fungicide-impregnated filter disks on the adaxial or abaxial leaf surface of detached leaves for 10 min and then incubating for 48 h before inoculating the abaxial surface with conidia. Vapor redistribution used Teflon disks sprayed with fungicides and placed on the abaxial leaf surface of detached leaves 48 h before inoculation. Disease development was rated 10 days later. Translaminar movement through calyptra was tested using flowering potted vines. All fungicides tested redistributed through at least one mechanism. Fungicide timing at critical phenological stages (early, mid, and late bloom) was assessed in small plots of cultivar Pinot noir vines. The application of trifloxystrobin, quinoxyfen, or fluopyram at different bloom stages showed that applications initiated at end of bloom resulted in the lowest berry infection probabilities of 0.073, 0.097, and 0.020, respectively. The results of this study suggest that integrating two carefully timed applications of redistributing fungicides initiated at end of bloom into a fungicide program may be an effective strategy for wine grape growers in western Oregon to produce fruit with low GPM infection.
葡萄白粉病(GPM)杀菌剂方案包括 5 至 15 次施药,具体取决于地区或市场,以试图达到市场所要求的高水果质量标准。了解杀菌剂如何重新分配以及将杀菌剂靶向到关键作物物候阶段,可以改善对葡萄串的杀菌剂保护。本研究评估了用于防治 GPM 的主要杀菌剂标签的杀菌剂在葡萄藤中的重新分配情况。通过将浸有杀菌剂的过滤盘置于离体叶片的叶面(叶背或叶腹)上 10 分钟,然后在接种叶背表面分生孢子之前再孵育 48 小时,来检查跨角质层和木质部的重新分配。使用喷涂有杀菌剂的特氟隆圆盘进行气相重新分配,并将其置于离体叶片的叶背表面 48 小时前进行接种。10 天后对发病情况进行评级。使用开花盆栽葡萄藤测试通过花帽进行的跨角质层运动。所有测试的杀菌剂都通过至少一种机制进行了重新分配。在小面积的黑比诺葡萄藤栽培品种中评估了关键物候阶段(早期、中期和晚期开花)的杀菌剂时间。在不同开花阶段施用肟菌酯、喹氧灵或氟吡菌胺的结果表明,在开花末期开始的施药导致最低的浆果感染概率分别为 0.073、0.097 和 0.020。本研究结果表明,将两种精心定时的重新分配杀菌剂施药方案(即在开花末期开始的两次施药)整合到杀菌剂方案中,可能是俄勒冈州西部葡萄酒葡萄种植者生产低 GPM 感染果实的有效策略。